端粒酶逆转录酶基因启动子调控FADD表达载体的构建  

hTERT gene promoter modulated constrution of expressionvector of FADD

作　　者：张险峰[1] 江应安[1] 罗和生[1] 

机构地区：[1]武汉大学人民医院,430061

出　　处：《国际消化病杂志》2008年第3期253-255,共3页International Journal of Digestive Diseases

基　　金：国家自然科学基金资助项目(30471690)

摘　　要：目的构建人端粒酶逆转录酶(hTERT)基因启动子调控FADD的特异表达载体。方法利用PCR技术克隆hTERT基因启动子,FADD基因片段,并将其插入带有绿色荧光蛋白(GFP)基因的表达载体中。结果hTERT基因启动子调控FADD表达载体经酶切鉴定含有人端粒酶逆转录酶基因启动子序列。结论hTRET基因启动子具有肿瘤特异性,构建hTERT基因启动子调控FADD表达载体可能是一种新型、有希望的肿瘤治疗的途径。Objective To construct FADD expression vector modulated by human telomerase reserve transcriptase gene promoter, Methods Genomic fragment of hTERT promoter and FADD （Fas-associated death domain proteln）were amplified by RT-PCR and inserted into the vector with GFP genomic fragment. The recombinant plasmid was identified by PCR and restriction endonuclease . The genomlc fragment of hTERT promoter and FADD were determined and analyzed by DNA sequencing, Results Sequencing and restriction endonuclease digestion demonstrated that the fragment was cloned and included human telomerase reserve transcriptase gene promoter sequence, Conclusion The hTERT gene promoter has the tumor specificity, suggesting that the specific expression vector modulated by hTERT gene promoter may be a novel and promising approach to the tumor treatment.

关 键 词：人端粒酶逆转录酶 启动子 基因治疗 表达载体 

分 类 号：R346[医药卫生—基础医学]