柔嫩艾美耳球虫免疫期间鸡盲肠扁桃体γ干扰素表达动态的检测  

Dection of Kinetic Expression of IFN-γ in Chicken Cecal Tonsil During Eimeria tenella Immunization

作　　者：王彩霞[1] 徐赓[1] 王黎霞[1] 张鹏[1] 安健[1] 

机构地区：[1]北京农学院动物科学技术系,北京102206

出　　处：《动物医学进展》2008年第6期5-10,共6页Progress In Veterinary Medicine

基　　金：北京市教委资助项目(KM20071002001);北京市属市管高校人才强教计划项目(PXM2007-014207-044539)

摘　　要：根据GenBank鸡β-actin、IFN-γ的基因序列设计引物,应用逆转录-聚合酶链反应(RT-PCR)技术克隆获得β-actin和IFN-γ基因,采用β-actin为内参的半定量方法检测IFN-γ mRNA在鸡柔嫩艾美耳球虫免疫前后不同时间的表达情况,以探讨IFN-γ的表达动态与柔嫩艾美耳球虫免疫的关系。结果显示,IFN-γ在两次免疫期间的表达量整体上呈现双峰模式,首免后第7天达到一个高峰,二免后第7天达到另一个高峰。According to chicken β-actin and IFN-γ gene sequences in GenBank, primers were designed, the gene sequences were amplificated by reverse transcription-polymerase chain reaction （RT-PCR） from cecal tonsil of chicken experimentally immunized by Eimeria tenella. The expression level of IFN-γ mRNA in different time after immunization was detected by semi-quantitative RT-PCR with β-actin gene as intra-reference to study the relatioship between E. tenella immunization and the kinetic expression of IFN-γ gene in chicken cecal tonsil. The result showed that the level of expression of IFN-γ gene reached 1^st peak on day 7 post-1^st -immunization,and reached 2^nd peak on day 7 posr-2^nd-immunization.

关 键 词：盲肠扁桃体 Β-肌动蛋白 Γ干扰素 逆转录-聚合酶链反应 鸡 

分 类 号：S852.723[农业科学—基础兽医学]