应用正交设计探讨肾虚胚胎着床障碍小鼠病症结合模型制作中药物的最佳配比  

Study on the pharmaceutical optimal proportion in the mouse model of integrated disease and syndrome of embryo implantation dysfunction with kidney asthenia by orthogonal design

作　　者：徐珉[1] 董燕[1] 黄健玲[1] 李丹颜 邓雪梅[1] 陈梅芬[1] 钟志勇[2] 黄小琼[2] 

机构地区：[1]广州中医药大学第二临床医学院妇科,广东广州510120 [2]广东省医学实验动物中心,广东佛山510180

出　　处：《新乡医学院学报》2008年第4期328-332,共5页Journal of Xinxiang Medical University

基　　金：国家自然基金资助课题(编号:30672707);广东省自然基金资助课题(编号:06024113)

摘　　要：目的探索羟基脲及米非司酮在肾虚胚胎着床障碍小鼠病症结合模型制作中的最佳配比。方法以羟基脲、米非司酮作为影响因素,选择L9(34)表安排实验,观察羟基脲及米非司酮不同剂量配比组合对小鼠肾虚胚胎着床障碍模型的影响。结果研究范围内,当羟基脲因素A取1水平、米非司酮因素B取1水平时(AⅠBⅠ),妊娠第5天、第8天时子宫内胚胎着床点数、子宫指数是最少的。在肾虚胚胎着床障碍小鼠模型的制作中,米非司酮的贡献度最大,羟基脲的贡献度次之,一定程度上,胚胎的着床点和子宫指数与羟基脲和米非司酮的用量成反比。结论综合多项指标,在研究范围内,AⅠBⅠ较为合适作为肾虚胚胎着床障碍小鼠模型用于药物干预研究。Objective To ascertain the optimal proportion of hydroxycarbamide and miferristone in the mouse model of integrated disease and syndrome of embryo implantation dysfunction （EID） with kidney asthenia syndrome. Methods Taken hydroxycarbamide, miferristone as influencing factors, according to the L9 （3^4 ） orthogonal array,mathematical model was erected to observed the effects of different combination of hydroxycarbamide and miferristone in the mouse model of EID with kidney asthenia. Results Within the scope of the study, when hydroxyurea was at 1 level and miferristone was at 1 level, the point number of embryo implantation in the uterus cavity, the index of uterus, ovaries and kidney, and the number of autonomic activities was the least in the fifth and eighth day of pregnancy. During construction of the mouse model of EID with kidney asthenia,miferristone took the greatest contribution, and hydroxycarbamide followed it. To a certain extent, the point number of embryo implantation and the uterus index was in inverse proportion to the dose of hydroxycarbamide and miferristone. Conclusion Integrating a number of indicators,within the scope of the study, A Ⅰ B Ⅰ was more appropriate to be used as the model of EID with kidney asthenia syndrome for further drug intervention study.

关 键 词：胚胎着床障碍 病症结合模型 正交设计 方差分析 

分 类 号：Q95-3[生物学—动物学]