共基质和无机盐对原油降解菌株降解原油效果的影响  

Effects of Co-Substrates and Inorganic Salts on Degradation of Crude Oil by Oil Degrading Bacteria

作　　者：刘晓春[1] 阎光绪[1] 郭绍辉[1] 陈春茂[1] 

机构地区：[1]中国石油大学(北京)化学科学与工程学院,北京102249

出　　处：《化工环保》2008年第3期218-221,共4页Environmental Protection of Chemical Industry

基　　金：中国石油天然气集团公司科学研究与技术开发项目(05D40102)

摘　　要：从大港油田石油污染土壤中分离筛选出1株原油降解菌X3,对原油的降解率达72.6%,经鉴定X3菌株属于假单胞菌属(Psedomonas)。利用生物摇床对X3菌株降解原油的实验发现,共代谢基质α-乳糖对X3菌株降解原油有促进作用,可使原油降解率提高到80.3%;而葡萄糖和蔗糖对X3菌株降解原油有抑制作用。Fe2+对X3菌株的降解原油也有促进作用,在α-乳糖和Fe2+的共同作用下,X3菌株对原油的降解率可达82.3%;K+和Mg2+对X3菌株降解原油则有抑制作用。在FeSO4质量浓度为0.2～0.3mg/L时,X3菌株对原油的降解率最高,FeSO4质量浓度继续增加,X3菌株对原油的降解率下降。One bacterial strain, designated as X3 which has the capability of degrading crude oil, was isolated from Dagang oil-field. The degradation rate of crude oil is 72.6%. It is identified that the strain X3 belongs to Psedomomas sp. The tests for crude oil degradation by the strain X3 were carried out under shaking-aeration condition. It is found that： Lactose has the effect of promoting the activity of the strain X3, and the degradation rate of crude oil is increased to 80.3% ; Glucose and sucrose have the effects of inhibiting the activity of the strain X3; Fe2＋ can also increase the crude oildegrading ability of the strain X3, the degradation rate of crude oil can come up to 82.3% under the synergistic interaction of lactose and Fe2＋ ; K ＋ and Mg2 ＋ will decrease the degrading ability of the strain X3; The degradation rate of crude oil is the highest when the concentration of FeSO4 is 0. 2 - 0.3 mg/L.

关 键 词：共基质 无机盐 原油 菌株 降解 

分 类 号：X53[环境科学与工程—环境工程]