芍药甙防治大鼠肝细胞体外损伤形态学及生物化学研究  

Rat's Hepatocellular Damage Protected and Treated by Paeoniflorin: Morphological and Biochemical Research in Vitro

作　　者：陈爽[1] 贲长恩[1] 杨美娟[1] 王德福[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中西医结合肝病杂志》1997年第4期217-220,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：国家教委博士点科研基金

摘　　要：目的:研究芍药甙对大鼠肝细胞体外损伤的防治作用。方法:采用D-氨基半乳糖和内毒素损伤大鼠原代培养肝细胞的方法造模,通过检测细胞形态变化和生化指标LDH、GOT、SOD等,观察芍药甙对其预防和治疗作用。结果:与各损伤组相对应的预防、治疗组,细胞表面结构、细胞核、内质网、高尔基复合体及线粒体、溶酶体等结构恢复正常。LDH、GOT、SOD也明显降低(P<0.05～0.01)。结论:芍药甙可通过增强肝细胞膜及溶酶体膜的稳定性,对肝细胞损伤起防治作用。Aim: To study the protective and therapeutic effects of paeoniflorin to rat's hepato-cellular damage in vitro. Methods: Primary culture damaged hepatocytes induced by D-galac-tosamine and/or endotoxin. The protective and therapeutic effects of paeoniflorin were observed by detecting the changes of cytomorphology and biochemical criterion such as lactic dehydrogenase (LDH). glutamic oxaloacetic transaminase (GOT), superoxide dismutase (SOD). Result: The structures of cell surface, caryon, endoplasmic reticulum , Golgi complex, mitochondrion , lysosome returned to normal in different protective and therapeutic groups which were corresponding to different damaged one. The expressions of LDH, GOT, SOD in different protective and therapeutic groups were obviously lower than those in damaged one (P<0.05-0.01). Conclusion: paeoniflorin can in(?)rease the stability of hepatocyte and lysosome membrane, so that it has the effects for protecting and treating hepatocellular damage.

关 键 词：芍药甙 中药 肝细胞 损伤 超微结构 生物化学 

分 类 号：R285.5[医药卫生—中药学] R256.405[医药卫生—中医学]