IGF-1对去卵巢大鼠骨密度及骨力学强度的影响  

Effects of exogenous IGF-1 on bone mineral density and biomechanical properties of ovariectomized rats

作　　者：赵荣兰[1] 刘新宇[2] 孙蓓[1] 梁东春[1] 郭刚[1] 张镜宇[1] 

机构地区：[1]天津医科大学代谢病医院内分泌研究所,卫生部激素与发育重点试验室,300070 [2]天津医科大学基础医学院生物化学教研室

出　　处：《国际内分泌代谢杂志》2008年第4期280-282,共3页International Journal of Endocrinology and Metabolism

基　　金：基金项目：天津市自然科学基金资助项目（033801611）

摘　　要：目的研究外源性胰岛素样生长因子-1（IGF-1）对去卵巢（OVX）骨质疏松大鼠骨密度、骨转换率、骨力学强度等方面的影响。方法对大鼠施行双侧卵巢摘除术，术后3个月以骨密度测定证实骨质疏松的存在后，随机分为5组，分别以生理盐水、甲状旁腺激素1—34及3种不同剂量IGF-1进行干预。同时设立生理盐水干预的假手术大鼠作为对照。8周后检测血清钙、磷、骨钙素水平及碱性磷酸酶活性；测定腰椎骨密度、股骨力学强度；组织学染色测定股骨远端骨皮质厚度。结果IGF-1虽未提高OVX大鼠腰椎骨密度却可以显著提高其股骨力学强度。血清学检测结果表明，IGF-1可降低血清钙、磷、骨钙素水平及碱性磷酸酶活性；组织学染色显示IGF-1可显著提高OVX大鼠股骨骨皮质厚度。结论IGF-1可增加OVX大鼠股骨的力学强度，此作用可能是通过改善骨结构而非提高骨密度所实现的。Objective To study the effects of exogenous IGF-1 on bone mineral density,bone turnover and bone biomechanical properties of ovariectomized （OVX） rats. Methods Sprague-Dawley rats were either ovariectomized（ n = 60） or sham operated（ n = 10）. Three months after the operation, the existence of osteoporosis in OVX rats was confirmed by bone mineral densitometry. OVX rats were randomly separated into 5 groups,subjected to the treatment of PTH1-34,three different-dosage of IGF-1 or normal saline（NS） re- spectively. Sham rats,treated with NS,was established as sham control. Eight weeks after the treatment,serum levels of Ca, P, steocalcin concentration and alkaline phosphatase （ALP） activity were assayed. The bone mineral density of lumbar spine and the mechanical strength of the femur were determined. The bone thick- ness of distal femur was determined by histological staining. Results IGF-1 did not improve the bone mineral density of lumbar spine in OVX rats, but significantly improved their mechanical strength. Serological test results showed that IGF-1 could lower serum calcium,phosphorus,calcium levels and ALP activity;histological staining showed that IGF-1 could significantly increase the femur bone thickness of OVX rats. Conclusion IGF-1 can increase the mechanical strength of the femur in OVX rats through reconstructing bone architecture rather than increasing bone mineral density.

关 键 词：胰岛素样生长因子-1 骨质疏松 骨密度 骨结构 

分 类 号：R580[医药卫生—内分泌]