超声破坏微泡促进骨骼肌血管新生的时间-效应关系研究  

The time-effect relationship of angiogenesis induced by ultrasound-mediated microbubble destruction in the skeletal muscle

作　　者：张亚兴[1] 王志刚[1] 许川山[1] 张群霞[2] 张茂惠[2] 谢昭鹏[2] 

机构地区：[1]重庆医科大学超声影像学研究所,400010 [2]重庆医科大学附属第二医院超声科,400010

出　　处：《中华超声影像学杂志》2008年第6期534-537,共4页Chinese Journal of Ultrasonography

基　　金：基金项目：国家高技术研究发展计划（863计划）（2006AA02Z4F0）,国家自然科学基金重点项目（30430230）及重庆市自然科学基金重点项目（CSTC）（2005BA5024）

摘　　要：目的探讨超声破坏微泡促进大鼠骨骼肌血管新生的时间-效应关系。方法将48只SD大鼠随机分为4组（超声破坏微泡组、单纯超声组、单纯微泡组、对照组），超声破坏微泡组经尾静脉输入自制的脂质全氟丙烷微泡造影剂0．5ml，同时用1．0MHz、2．0W／cm^2的超声在其大腿骨骼肌局部作用3min；单纯超声组仅在骨骼肌局部用同等的超声能量作用；单纯微泡组仅由尾静脉输入脂质全氟丙烷微泡造影剂0．5ml；对照组不作任何处理。实验结束后的第3、7、10、14、21、28d，各组分别取两只大鼠处死，取局部骨骼肌行石蜡切片HE染色观察显微结构的变化，CD34免疫组化计数微血管密度（MVD），酶联免疫吸附试验（ELISA）定量评价血管内皮生长因子（VEGF）的表达。结果超声破微泡组有较多的血管新生；单纯超声组新生血管较少；单纯微泡组和对照组几乎无血管新生。随着时间的变化，超声破坏微泡组的MVD值和VEGF表达在第10d达到高峰；单纯超声组的高峰出现在第14d。结论超声破坏微泡可刺激骨骼肌中内源性VEGF较快、较多地分泌，从而更好地促进新生血管生成。Objective To investigate the time-effect relationship of angiogenesis induced by ultrasound-mediated microbubble destruction in the skeletal muscle of rats. Methods Forty-eight healthy SD rats were divided into 4 groups： ultrasound-mediated microbubble destruction group, ultrasound only group,microbubble only group and control group. In ultrasound-mediated microbubble destruction group, microbubbles were injected by vein at a dose of 0.5 ml and the target skeletal muscle was radiated at 2.0 W/cm^2. In ultrasound only group, the target skeletal muscle was radiated at 2.0 W/cm^2. In microbubble only group,microbubbles were injected by vein at a dose of 0.5 ml. The control rats were without ultrasound radiation and microbubbles. On the 3rd, 7th, 10th, 14th, 21 st and 28th day after the ultrasound radiation, two rats in each group were sacrificed and the target skeletal muscle was harvested for HE staining to observe the microstructure of tissue, immunohistochemistry staining was used to count the microvessel density （MVD）, enzyme linked inmmunosorbent assay （ELISA） was used to detect the expression of vascular endothelial grouth factor （VEGF）. Results Angiogenesis was significant in ultrasound-mediated microbubble destruction group, but a little in ultrasound only group. There was not any angiogenesis in either microbubble only group or control group. MVD and VEGF expression of ultrasound-mediated microbubble destruction group arrived at a peak on the 10th day as well as on the 14th day of ultrasound group. Conclusions Ultrasound-mediated microbubble destruction can facilliate the endogenous secretion of VEGF quickly and more,and accelerate the angiogenesis in the skeletal muscle.

关 键 词：超声检查 微气泡 肌 骨骼 新生血管化 生理性 

分 类 号：R686[医药卫生—骨科学]