钙调素对梨自花及异花授粉后花柱自发荧光变化的影响  

The Variation of Style Auto-fluorescence after Calmodulin Treatment on Self-and Cross-pollination in Pyrus pyrifolia Nakai

作　　者：周建涛[1] 姜雪婷[1] 刘珠琴[1] 张绍铃[1] 

机构地区：[1]南京农业大学园艺学院,南京210095

出　　处：《园艺学报》2008年第6期781-786,共6页Acta Horticulturae Sinica

基　　金：高校博士学科点专项科研基金项目(B200523)

摘　　要：采用激光共聚焦显微镜研究了外源钙调素（CaM）及其抗血清对‘丰水’梨自花（不亲和）和异花（亲和）授粉后花柱自发荧光变化的影响。结果表明：授粉12h后，CaM处理后异花授粉花柱荧光值与未处理异花授粉花柱荧光值相比，花柱上部荧光值降低，下部升高；而CaM处理后自花授粉花柱自发荧光值除距柱头2500-3000μm外其余部分均高于未处理自花授粉花柱；CaM抗血清处理后异花授粉花柱荧光值除距柱头1000-2000μm外其余部分均高于未处理异花授粉花柱；CaM抗血清处理自花授粉花柱自发荧光与未处理自花授粉花柱相比，分布规律发生改变，最高值位于距柱头1000-1500μm处。授粉72h后CaM处理后异花授粉花柱荧光值高于未处理异花授粉花柱，最高值位于子房端；CaM处理后自花授粉与未处理自花授粉花柱自发荧光值相比，分布规律发生改变，最高值位于距柱头1500-2000μm处；CaM抗血清处理后异花及自花授粉花柱自发荧光与未处理异花及自花授粉花柱相比较，分布规律均发生了改变，最高值均位于0-500μm处。证实了外源CaM及其抗血清对自花、异花授粉的生理代谢过程均有影响。Confocal laser scanning microscopy（CLSM）were used to investigate the role of exogenous calmodulin（CaM）and anti-CaM serum in pear＇Hosui＇ style auto-fluorescence characterization after self-and cross-pollination for 12 h and 72 h.The results showed that the auto-fluorescence of cross-pollination topside style treated with CaM for 12 h reduced and the bottom increased,but the auto-fluorescence of self-pollination style treated with CaM increased except 2 500-3 000 μm behind stigma.Anti-CaM serum treated cross-pollination style increased the whole auto-fluorescence except 1 000-2 000 μm behind stigma and the tiptop of self-pollination after anti-CaM serum treatment moved to 1 000-1 500 μm behind stigma.After pollination 72 h,the style of cross-pollination treated with CaM increased its auto-fluorescence and the highest value was at ovary.Self-pollination style treated with CaM changed the distributing disciplinarian of auto-fluorescence,its tiptop were at 1 500-2 000 μm behind stigma.The highest auto-fluorescence of self-and cross-pollination treated with anti-CaM serum moved to 0-500 μm behind stigma.This primary evdience showed here confirmed that exogenous calmodulin and anti-CaM serum have effected on metablism of the self-and cross-pollination.

关 键 词：梨 钙调素 自交不亲和性 自发荧光 

分 类 号：S661.2[农业科学—果树学]