车轮梅茎段高效再生体系的建立  

Establishment of A Highly Efficient System of Shoot Regeneration from Stem Segment Explants of Rhaphiolepis indica Lindl.

作　　者：刘娟旭[1,2] 刘玲[1] 余义勋[1] 王静[1] 黄现宝[1] 

机构地区：[1]华南农业大学园艺学院,广州510642 [2]University of Florida, Institute of Food and Agricultural Sciences, Apopka, FL 32703-8504, USA

出　　处：《园艺学报》2008年第6期895-898,共4页Acta Horticulturae Sinica

基　　金：霍英东教育基金项目(104031);广东省自然科学基金项目(05300848)

摘　　要：以车轮梅(Rhaphiolepis indica Lindl.)茎段为外植体,探讨基本培养基种类、植物生长调节剂组合、蔗糖浓度和AgNO3等对茎段器官发生和植株再生的影响。结果表明:MS+6-BA2.0mg·L-1+NAA0.5mg·L-1+AgNO31.0mg·L-1+蔗糖40g·L-1培养基最适合不定芽的分化和增殖,不定芽分化率达90%以上,平均每外植体分化不定芽数达5.38个。不定芽可在1/2MS培养基中有效伸长,适宜的生根培养基为1/2MS+NAA1.0mg·L-1,生根率达到100%。An efficient plant regeneration protocol for Rhaphiolepis indica Lindl.was achieved via organogenesis direct formation on stem segment explants in vitro.Several factors affecting in vitro regeneration of R.indica,such as basic medium,different hormones combination,concentrations of sucrose and AgNO3,were examined.The optimum medium for shoot regeneration was MS medium containing 2.0 mg·L^-16-BA,0.5 mg·L^-1NAA,1.0 mg·L^-1AgNO3 and 40 g·L^-1sucrose.90% of adventitious shoot regeneration frequency with 5.38 shoots per explant was obtained.Excised shoots were effectively elongated in 1/2 MS medium.Elongated shoots of 3.0 cm rooted when they were transferred to 1/2MS medium containing 1.0 mg·L^-1 NAA after 40 days and developed into healthy plantlets,which resulted in a rooting rate of 100%.

关 键 词：车轮梅 茎段 植物生长调节剂 不定芽再生 

分 类 号：S685.12[农业科学—观赏园艺]