针刺足三里穴对冷应激性胃溃疡大鼠下丘脑与肾上腺SP和POMC表达的影响  

Effect of acupuncturing at the acupoint ST 36 on substance P and proopiomelanocortin expression in hypothalamus and adrenal in rats with coldrestraint stress-induced ulcer

作　　者：孙锦平[1] 王路宁[1] 柳国芳[1] 尹岭[2] 

机构地区：[1]青岛大学医学院附属医院急诊神经内科,山东省青岛市266003 [2]中国人民解放军总医院神经信息中心,北京市100853

出　　处：《世界华人消化杂志》2008年第15期1602-1606,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30171135~~

摘　　要：目的:研究针刺足三里穴对冷应激性急性胃溃疡的保护作用,以及应激大鼠下丘脑和肾上腺SP和POMCmRNA的表达情况.方法:大鼠22只随机分成3组:正常对照组(6只)、单纯应激组(8只)、针刺预防应激组(8只).针刺预防组先给于针刺足三里穴,再冷应激.胃黏膜溃疡指数和RT-PCR法研究针刺足三里对大鼠的冷应激性溃疡的保护作用和下丘脑、肾上腺的SP、POMC的表达,经图像分析系统照相并进行半定量.结果:与单纯应激组比较,针刺足三里穴可以明显减少冷应激造成的溃疡指数(9.75±1.91vs26.25±4.40,P<0.01),抑制应激造成的皮质醇分泌增加(66.83nmol/L±12.25nmol/Lvs104.38nmol/L±8.31nmol/L,P<0.01),上调下丘脑的SP的表达(1.02±0.42vs0.45±0.12,P<0.05),下调肾上腺SP的表达(1.88±0.82vs2.93±1.08,P<0.05);下丘脑POMC在应激过程中表达增高,针刺能抑制POMC的表达,刺预防应激组与单纯应激组相比差异显著(0.56±0.14vs0.82±0.19,P<0.01);肾上腺中无POMC表达,不参与应激过程.结论:针刺对冷应激溃疡具有保护作用,该种保护作用是通过对生理性上调下丘脑SP的表达,下调POMC的病理性表达及抑制肾上腺SP而实现的.AIM： To study the protective effect of acupuncturing at the acupoint ST 36 against cold-restraint stress-induced ulcer and the expression of substance P （SP） and proopiomelanocortin （POMC） associated with stress in hypothalamus and adrenal in rats. METHODS： Twenty-two rats were randomized into 3 groups： normal control group （n = 6）, stress group （n = 8）, acupuncturing group （n = 8）. The rats in the acupuncutring group received acupuncture at the acupoint ST 36 before cold-restraint stress. Ulcer index and serum cortisol level were used to evaluate the protective effect of acupuncturing at the acupoint ST 36; reverse transcriptionpolymerase chain reaction （RT-PCR） was used to detect the expression of SP and POMC in hypothalamus and adrenal, and the images were analyzed with semi-quantitative method. RESULTS： The ulcer index and serum cortisol level in the acupuncturing group were significantly lower than those in the stress group （9.75 ± 1.91 vs 26.25 ± 4.40, P 〈 0.01; 66.83 nmol/L ＋ 12.25 nmol/L vs 104.38 nmol/L ± 8.31 nmol/L, P 〈 0.01）. The expression of SP was up-regulated in hypothalamus （1.02 ± 0.42 vs 0.45± 0.12, P 〈 0.05） but down-regulated in adrenal （1.88 ± 0.82 vs 2.93± 1.08, P 〈 0.05） in the acupuncturing group as compared with that in the stress group. Acupuncturing at the acupoint ST 36 inhibited the sress-induced POMC expression in hypothalamus （0.56 ± 0.14 vs 0.82 ± 0.19, P 〈 0.01）. There was no POMC expression in adrenal in the rats with sress-induced ulcer. CONCLUSION： Acupuncturing at acupoint ST 36 can protect gastric mucosa against coldrestraint stress-induced ulcer by up-regulating SP expression in hypothalamus and down-regulating POMC expression in hypothalamus and SP expression in adrenal.

关 键 词：应激性溃疡 P物质 前阿黑皮素原 逆转 录聚合酶链反应 

分 类 号：R245[医药卫生—针灸推拿学]