铁过载促进小鼠肝组织发生蛋白质酪氨酸硝化  

Iron-overloading Promotes Protein Nitration in Mouse Liver

作　　者：李海玲[1] 张燕[1] 卢乃浩[1] 徐辉碧[1] 高中洪[1] 

机构地区：[1]华中科技大学化学与化工系生物无机化学与药物湖北省重点实验室,武汉430074

出　　处：《中国生物化学与分子生物学报》2008年第6期569-573,共5页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金(No.30670481);教育部“新世纪优秀人才支持计划”(NCET-05-0649);上海高校E研究院基金(E-04010)资助项目~~

摘　　要：蛋白质酪氨酸硝化是一种蛋白质翻译后的修饰,其存在会影响酶的催化活性,细胞信号转导和细胞骨架结构.在铁过载情况下,存在引起蛋白质酪氨酸硝化的有利环境,但目前尚无实验证实.本文运用腹腔注射右旋糖苷铁造成小鼠铁过载模型,通过免疫印迹法发现,在铁过载情况下,肝中诱导型一氧化氮合酶表达显著高于正常对照小鼠;铁过载小鼠肝中总体蛋白质硝化程度高于正常小鼠;铁过载引起的蛋白质酪氨酸硝化有一定的选择性,在铁过载小鼠肝中发现一些新的被硝化蛋白质条带(约57kD、35kD).上述结果证实,铁过载会促进肝蛋白质酪氨酸硝化.Protein nitration is a post-translational modification which will induce the changes of protein catalytic activities,cell signaling and cell skeletal structure.Under iron-overloaded condition,there are advantageous features to promote protein nitration.However,up to date,direct evidence on the role of iron-overloaded elevating hepatic protein nitration is little.In this article,iron-overloaded mice were obtained by peritoneal injection with iron-dextran.Western blotting indicated that iron-overloading could increase the expression of inducible nitric oxide as well as the extent of protein tyrosine nitration in liver,and some new protein nitration bands were found（about 57 kD and 35 kD）,indicating that iron overloading caused the selectivity of protein nitration in vivo.

关 键 词：铁过载 肝损伤 蛋白质酪氨酸硝化 诱导型一氧化氮合酶 

分 类 号：Q51[生物学—生物化学]