骨髓源神经干细胞移植改善一氧化碳中毒大鼠脑功能  

Cerebral functional improved in rats with severe acute monoxide poisoning by transplantion of neural stem-like cells derived from bone marrow mesenchymal stem cells

作　　者：马岳峰[1] 蒋国平[1] 高建平[1] 徐永山[1] 江观玉[1] 

机构地区：[1]浙江大学医学院附属第二医院急诊中心,杭州310009

出　　处：《中华急诊医学杂志》2008年第6期583-586,共4页Chinese Journal of Emergency Medicine

基　　金：教育部高等学校博士学科点专项科研基金（20040335126）

摘　　要：目的探讨骨髓间充质干细胞（bone marrow mesenchynal stem cells，BMSCs）来源的神经干样细胞（neural stem-like cells，MS-NSCs）移植治疗对急性重症一氧化碳中毒大鼠脑功能的影响。方法40只SD（Sprague Dawley）大鼠随机分成正常对照组（A）、中毒对照组（B）、BMSCs治疗组（C）、MS-NSCs治疗组（D）。体外分离大鼠BMSCs，定向诱导为MS-NSCs，BrdU标记．建立中毒模型并复苏24h后，将治疗细胞经左颈内动脉移植入中毒大鼠脑内，以神经功能严重度评分和Morris水迷宫试验评价脑功能的改善程度。结果5周后神经功能严重度评分各组间差异无统计学意义，Morris水迷宫试验B组、C组、D组较A组差异具有统计学意义（P〈0．01），C组、D组较B组差异具有统计学意义（P〈0．05），D组较C组差异具有统计学意义（P〈0．05）。结论MS-NSCs细胞移植能够促进认知记忆功能的恢复，且这种作用较BMSCs更明显，提示前者可能是更佳的种子细胞。Objective To investigate cerebral functional remodeling of the rat with severe acute carbon monoxide poisoning transplanted with neural stem-like cells derived from bone marrow mesenchymal stem cells （MS-NSCs）. Method Forty Sprague-Dawley rats weighing 200 - 250 g were divided randomly into 4 groups：the normal control group, the poisoning control group, the bone marrow mesenchymal stem cells（BMSCs） transplantation group and the MS-NSCs transplantation group. BMSCs were harvested from whole bone marrow in vitro, then were differentiated into MS-NSCs under certain growth factors cocktail, and were followed by BrdU labelhng. Twen- ty-four hours after poisoning, the seed cells were infused into brain via left internal carotid and the functional remodeling of cerebrum was assessed by neurological severity score（NSS） and Morris water maze（MWM） tests. Resuits There was no significant differences in NSS test between groups after transplantation. However, the differences in MWM test were very significant between 5 weeks after transplantation （ P 〈 0. 01 ）. Conclusions Transplantation of MS-NSCs may improved cerebral function of rats after severe acute CO poisoning. Moreover, the cultured and idfferentiated MS-NSCs induced in vitro preliminarily is potentially more efficient than directly transplanted BMSCs without culture and differentiation.

关 键 词：骨髓间充质干细胞 神经干样细胞 分化 细胞移植 中毒 一氧化碳 

分 类 号：R595.1[医药卫生—内科学] R651.2[医药卫生—临床医学]