δ阿片受体激动剂DADLE对全脑缺血-再灌注大鼠血清S-100B蛋白的影响  

Influence of the δ-opioid receptor DADLE on serum protein S-100B after global cerebral ischemia and reperfusion in rats

作　　者：韩洁韵[1] 梁子敬[1] 范彦琦[1] 

机构地区：[1]广州医学院第一附属医院急诊科,广州510120

出　　处：《中华急诊医学杂志》2008年第6期618-621,共4页Chinese Journal of Emergency Medicine

摘　　要：目的通过比较血清S-100B蛋白水平来观察δ阿片受体激动剂DADLE对大鼠全脑缺血-再灌注损伤的影响，从而探讨DADLE在脑复苏中的神经保护作用。方法用二血管阻断加低血压法制作大鼠全脑缺血．再灌注模型。50只SD大鼠随机分为五组，每组各10只；假手术组：不制模不干预；模型组：单纯制作全脑缺血-再灌注模型；DADLE预处理组：在缺血前给予DADLE；DADLE缺血后处理组：在缺血后给予DADLE；DADLE再灌注期处理组：在再灌注早期给予DADLE。实验结束后分别取血，用酶联免疫检测法（ELISA）测定大鼠血清S-100B蛋白水平。数据用均数±标准差（-↑x±s）表示，统计分析采用单因素方差分析，两两组间均数之间的比较采用SNK法，以P〈0．05为差异具有统计学意义。结果假手术组的血清S-100B蛋白水平为（475．56±41．93）pg／ml，模型组和DADLE处理各组的血清S-100B蛋白水平均比假手术组高（P〈0．05），DADLE处理各组的血清S-100B蛋白水平均比模型组低（P〈0．05），DADLE处理各组间的血清S-100B蛋白水平差异无统计学意义（P〉0．05）。结论δ阿片受体激动剂DADLE对大鼠全脑缺血-再灌注损伤有保护作用。Objective To observe the influence of δ-opioid receptor DADLE on global cerebral ischemia and reperfusion in rats with change in serum protein S-100B level and to explore the neuroprotective effect of DA- DLE during cerebral resuscitation. Method The model of global cerebral ischemia and reperfusion was induced by bilateral common carotid -artery occlusion combined with hypotension. Fifty SD rats were randomly divided into five groups： sham operation group, model group, DADLE pretreated group, DADLE treated postischemia group, DADLE treatment during reperfusion group （ n = 10 for each group）. In sham operation group, the rats were operated without ischemia and treatment; in model group, rats had global cerebral ischemia and reperfusion model up without any treament; in DADLE pretreated group, rats received DADLE before ischemia; in DADLE treatment postischemia group, rats had DADLE immediately after ischemia; in DADLE treatment during reperfusion group, rats got DADLE during early reperfusion. After the establishment of model, serum protein S- 100B was measured by using ELISA. One-way analysis of variance and SNK test were used for comparison between groups. Results The serum protein S-100B level was （475.56± 41.93） pg/ml in sham operational group and that was much lower than that in model group and DADLE treatment groups. While the levels of serum protein S-100B in all DADLE treatment groups were reduced significantly in comparison with model group. There were no differences in the levels of serum protein S-100B between DADLE treatment groups. Conclusions The δ-opioid receptor DADLE exerts neuroprotective effects on global cerebral ischemia and reperfusion in rots.

关 键 词：Δ阿片受体 全脑缺血-再灌注 S-100蛋白 

分 类 号：R285.5[医药卫生—中药学]