光和糖对水稻Rubisco活化酶基因表达的影响  

The Influence of Light and Sugar on the Gene Expression of Rubisco Activase in Rice

作　　者：唐如航[1] 贾军伟[1] 李立人[1] 

机构地区：[1]中国科学院上海植物生理研究所,上海200032

出　　处：《植物生理学报（0257-4829)》1997年第4期337-341,共5页Acta Phytophysiologica Sinica

基　　金：国家自然科学基金

摘　　要：水稻黄化苗在光照2h内其Rubisco。活化酶的mRNA和蛋白量明显增加，然后维持在相对稳定的水平。光对水稻Rubisco活化酶的基因表达的诱导作用主要在转录水平上。Rubisco活化酶主要在绿叶中表达，这与Rubisco基因表达的器官特异性完全一致。用等渗葡萄糖喂养成熟的水稻叶片1h，促使水稻Rubisco大、小亚基和Rubisco活化酶可翻译mRNA含量下降。同样蔗糖对Rubisco小亚基和Rubisco活化酶的表达也有抑制，其作用弱于葡萄糖。Northern blot analysis of Rubisco activase mRNA showed (Fig. 1 ) thatRubisco activase gene is expressed mainly in green leaves, which is similar to the cases of rbsL and rbcS genes.The translatable mRNA content of Rubisco activase increased significantly with time f illumination in etilated leaves up to 2 hours, and then kept at a stable level (Fig. 2). The transcription of Rubisco activase gene was mre sensitive to light than that of rbcL and rbcS genes. Analysis by Western blot showed that the gene expressin of Rubisco activase is regulated by light at the transcriptional level (Fig. 3).When mature leaves were fed with glucose, the translatable mRNA's for rbcS, rbcL and rca declined by 60%,45% and 24% respectively after 1 h (Fig. 5 ). However, the contents of translatuble mRNA in mature rice leaves treated with mannitol were almost unchanged. The inhibition of transcription of rca gene by glucose was higher than that by sucrose (Fig. 4 ).The Dot blot results (Fig. 6 ) showed that the gene expression of rca in immature leaves was not repressed, which was different from that in mature leaves.

关 键 词：水稻 光 糖 RUBISCO活化酶 基因表达 

分 类 号：S511.01[农业科学—作物学]