花叶锦带花组织培养的研究  

Stem-segment Culture and Propagation of Weigela florida cv.Variegata

作　　者：侯淑婧[1] 沈漫[2] 高遐虹[2] 沈红香[2] 董清华[2] 王志忠[2] 

机构地区：[1]新疆农业大学林学院,乌鲁木齐830052 [2]北京农学院植物科学技术系,北京102206

出　　处：《北京农学院学报》2008年第2期28-31,共4页Journal of Beijing University of Agriculture

基　　金：北京市教委资助项目(项目编号:KM200610020008)

摘　　要：以花叶锦带花的幼嫩茎段为外植体,进行组织培养与快速繁殖试验。研究结果表明:(1)初代培养的适宜培养基为MS+6-BA 0.50 mg/L+IBA 0.05 mg/L,附加480 mg/L氨苄青霉素钠,可有效抑制内生菌的污染,抑菌率达93.3%;(2)适宜增殖的培养基为MS+6-BA 0.50 mg/L+NAA 0.05 mg/L,增殖系数达6.70,苗健壮,无玻璃化现象;(3)生根最佳培养基为1/2MS+IBA 0.50 mg/L,生根率达98%,根系粗壮。The young stem-segment with axillary buds was used as the explant, the propagation by tissue culture of Weigela florida cv. Variegata was studied. The results showed that the media coutaining 480mg/L sodium ampicillin was helpful to reduce the propagation of plant endophyte. The appropriate media for germination of the axillary buds was MS added 6-BA （0.50mg/L） and IBA （0. 05mg/L）. The subculture media for plant differentiation was MS added 6-BA （0.50mg/L） and NAA （0.05mg/L）, the high coefficient of propagation was 6.70. At the same time, the plants cultured in tube had not the vitricplant phenomena. For plant rooting, the appropriate media was 1/2MS added IBA （0.50mg/L）, which could brought on the high rooting rate at 98%.

关 键 词：花叶锦带花 组织培养 快速繁殖 

分 类 号：S682[农业科学—观赏园艺]