大鼠心肌膜、肺、肠黏膜微血管内皮细胞静息膜电位的检测  

Measurement of the Resting Membrane Potential of Myocardium,Pulmonary,Intestinal Microvascular Endothelial Cells in Rat

作　　者：于志强[1] 索占伟[2] 张涛[1] 刘烨[1] 花宝光[3] 胡格[2] 段慧琴[2] 陈武[2] 穆祥[1] 

机构地区：[1]北京农学院兽医学(中医药)北京市重点实验室,北京102206 [2]北京农学院动物科学技术系,北京102206 [3]北京农学院生物技术系,北京102206

出　　处：《北京农学院学报》2008年第2期32-34,共3页Journal of Beijing University of Agriculture

基　　金：北京市自然科学基金重点项目(项目编号:6061001);北京市教委"学术创新团队"(项目编号:5090245)

摘　　要：比较大鼠心肌膜、肺、肠黏膜微血管内皮细胞静息膜电位,为内皮细胞电生理特性研究提供数据。用植块法培养大鼠心肌膜、肺、肠黏膜微血管内皮细胞,采用膜片钳放大器全细胞记录模式,分别测定3种细胞的静息膜电位。结果表明:心肌膜微血管内皮细胞的静息膜电位为(-24±4)mV;肺微血管内皮细胞的静息膜电位为(-27±6)mV;肠黏膜微血管内皮细胞静息膜电位为(-18±4)mV。3种不同组织来源的微血管内皮细胞在静息膜电位上存在异质性。To measure the resting membrane potential of cultured myocardium, pulmonary, intestinal microvascular endothelial cells in rat, Rat myocardium, pulmonary and intestinal microvascular endothelial cells were isolated and cultured by explant methods. The resting membrane potential were recorded by whole-cell patch clamp technique. The resting membrane potential of myocardium, pulmonary, intestinal microvascular endothelial cells were （ - 24 ±4）, （ - 27 ± 6） and （- 18±4） mV. There were significant differences among the resting membrane potential of the cultured microvascular endothelial cells from different tissues.

关 键 词：微血管内皮细胞 膜片钳 静息膜电位 

分 类 号：S853.74[农业科学—临床兽医学]