The Determination of Canthaxanthin in Feed Ingredients by High Performance Liquid Chromatography  

作　　者：ZHANG Hua YANG Xin MA Ying DONG Aijun XU Weili ZHANG Yingchun 

机构地区：[1]School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China

出　　处：《Journal of Northeast Agricultural University(English Edition)》2008年第2期37-40,共4页东北农业大学学报（英文版）

摘　　要：A series of studies were conducted to establish a methodology for the accurate and efficient determination of canthaxanthin in feed ingredients. Agilent ZORBAX Eclipse DB-C 18 analytical column （4.6×150 mm, 5μm） was used and kept at 25 ℃. The mobile phase was acetonitrile ： methanol （v/v）=95 ： 5, and eluted compounds were detected by DAD absorbance （470 nm）. The flow rate was maintained at 1.0 mL·min^-1. The response of the method was linear over the range 1.0-20.0 μg·mL^-1 canthaxanthin assay solution （R^2=0.9998）. Recovery assays done with standard canthaxanthin to feed ingredient resulted in an average recovery of 103%. Variation coefficient was less than 3.53%. This method is proved to be simple, precise, sensitive and reproductive.A series of studies were conducted to establish a methodology for the accurate and efficient determination of canthaxanthin in feed ingredients. Agilent ZORBAX Eclipse DB-C 18 analytical column （4.6×150 mm, 5μm） was used and kept at 25 ℃. The mobile phase was acetonitrile ： methanol （v/v）=95 ： 5, and eluted compounds were detected by DAD absorbance （470 nm）. The flow rate was maintained at 1.0 mL·min^-1. The response of the method was linear over the range 1.0-20.0 μg·mL^-1 canthaxanthin assay solution （R^2=0.9998）. Recovery assays done with standard canthaxanthin to feed ingredient resulted in an average recovery of 103%. Variation coefficient was less than 3.53%. This method is proved to be simple, precise, sensitive and reproductive.

关 键 词：HPLC FEED CANTHAXANTHIN carotenoids 

分 类 号：O657.72[理学—分析化学] S816.7[理学—化学]