应用光镜和电镜对病虾组织细胞病理变化的观察与分析  

Observation and Analysis of Histo-and Cyto-pathological Changes of Diseased Shrimp with Light and Electron Microscopy

作　　者：黄灿华[1] 张建红[1] 高玮[1] 石正丽[1] 

机构地区：[1]中国科学院武汉病毒研究所,武汉430071

出　　处：《中国病毒学》1997年第4期364-370,共7页Virologica Sinica

基　　金：国家"八五"攻关资助

摘　　要：应用光镜和电子显微镜技术比较研究正常与发病的中国对虾7种组织细胞病理变化，结果显示，病毒侵染后，对虾组织病理变化主要集中在消化系统的肝胰腺、中肠、胃等组织。在光镜下，可见消化道内壁上皮组织广泛受损，细胞大量坏死或空泡化；电镜下可见主要的细胞器如线粒体嵴大量断裂、粗面内质网严重扩张、溶酶体增生，病变细胞内出现大量变性的膜性结构等。观察结果为弄清对虾病毒引起的病症及致病机理打下基础。With light and electron microscopy techniques, the pathological changes of seven tissues of Panaeid chinensis were studied. The major pathological changes mainly took place in the tissues of digestive system: hepatopancreas, midgut and stomach etc. Under light microscope, it was found that the epithelia of inner digestive tract were widely damaged, most epithelial cells were necrotic or vaculated.While under electron microscope, apparent pathoogical changes of main organelles could be observed in diseased shrimps. For example, most cristae of mitochondrias broken, rough endoplasmic reticulum greatly expanded, lysosomes and various mutilayered membrane structures proliferated etc.

关 键 词：中国对虾 组织细胞病理 光镜 电镜 

分 类 号：S945.26[农业科学—水产养殖]