免疫荧光检测HIV-1抗体方法的建立和应用  

The Establishment and Application of Immunofluorescence Assay for Detection of Antibodies to HIV-1

作　　者：李敬云[1] 王宏霞[1] 鲍作义[1] 

机构地区：[1]北京军事医学科学院微生物流行病研究所,北京100071

出　　处：《中国病毒学》1997年第4期371-373,共3页Virologica Sinica

摘　　要：Using the mixture of HIV -1 infected and no - infected HeLa CD4+ cells, the antigen slides were prepared and the immunofluorescence assay for detection of antibodies to human immunode-ficiency virus type 1 was established. Seventy - seven serum samples including the national stan-dard reference of HIV antibody reagents were tested. The sensitivity and specificity were 96. 4 %and 100%, respectively. Compared with the confirmation test of Western Blot, the coincident rate was 92. 6 %. The results indicated that the specificity of immunofluorescence assay was high, and the positive predicted value was 100%, so it can be used as one of the confirmatory methods for HIV -1 antibody.Using the mixture of HIV -1 infected and no - infected HeLa CD4+ cells, the antigen slides were prepared and the immunofluorescence assay for detection of antibodies to human immunode-ficiency virus type 1 was established. Seventy - seven serum samples including the national stan-dard reference of HIV antibody reagents were tested. The sensitivity and specificity were 96. 4 %and 100%, respectively. Compared with the confirmation test of Western Blot, the coincident rate was 92. 6 %. The results indicated that the specificity of immunofluorescence assay was high, and the positive predicted value was 100%, so it can be used as one of the confirmatory methods for HIV -1 antibody.

关 键 词：免疫荧光 艾滋病毒-1 感染 抗体检测 

分 类 号：R512.910.4[医药卫生—内科学] R373.9[医药卫生—临床医学]