尼尔雌醇作用下去势雌兔骨组织形态计量学改变  

Histomorphometric Study of the Effect of Nylestriol on Bone Turnover in Ovariectomized Rabbits

作　　者：史炜镔[1,2] 杜宁[1,2] 符诗聪[1,2] 曲克服[1,2] 李国衡[1,2] 

机构地区：[1]上海第二医科大学附属瑞金医院伤骨科 [2]上海市伤骨科研究所

出　　处：《中华妇产科杂志》1997年第9期544-547,共4页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的：评估尼尔雌醇对绝经后骨质疏松症的疗效，并探讨其机理。方法：将３０只雌性新西兰兔随机分成３组（每组１０只）：治疗组［摘除卵巢＋尼尔雌醇（每月２ｍｇ）］、模型组（摘除卵巢）和正常对照组（假手术），６个月后处死取材，并在处死前行四环素标记，ＶＩＤＡＳ图象分析系统下作骨组织形态计量学测定。结果：治疗组的单位体积骨小梁骨量（ＢＶ／ＴＶ）、相对类骨质量（ＯＶ／ＢＶ）、成骨细胞指数（ＯＢＩ）和组织水平的骨形成速率［ＢＦＲ（Ｔ）］明显高于模型组和（或）正常对照组。结论：尼尔雌醇能增加成骨细胞数量，可能是一种成骨细胞募集和（或）增殖的刺激剂。Objective: To assess the effect of Nylestriol on postmenopausal osteoporosis in an attempt to reveal its mechanism. Methods: Thirty seven month old female New Zealand white rabbits were randomized into 3 groups (10 rabbits per group): treatment group [ovariectomized (OVX)+nylestriol (2 mg, monthly)], OVX control group (OVX+vehicle) and normal control group (sham operation +vehicle). Six months later, all rabbits were subjected to tetracycline labelling and then were killed. Under VIDAS system, bone turnover was assessed by bone histomorphometry. Results: Bone volume trabecular (BV/TV),relative osteoid volume (OV/BV), osteoblastic index (OBI) and bone formation rate at tissue level increased in treatment group compared with OVX control group and (or ) sham control group. Conclusions: Nylestriol can increase the number of osteoblast, which resulted in more bone matrix production and consequently converted the bone turnover of OVX rabbits to a positive balance situation. It indicated that Nylestriol was probably a stimulator for osteoblast recruitment and (or ) proliferation.

关 键 词：骨密度 骨质疏松 绝经后 尼尔雌醇 形态计量学 

分 类 号：R588[医药卫生—内分泌] R681[医药卫生—内科学]