依那普利对高肺血流量大鼠血管紧张素Ⅱ、血浆一氧化氮的影响  

Effect of Enalapril on AngiotensinⅡ and Serum Nitric Oxide in Rats with High Pulmonary Blood Flow

作　　者：周菁[1] 严文华[2] 吕海涛[2] 文德[2] 葛薇[2] 

机构地区：[1]苏州大学附属儿童医院呼吸科,江苏苏州215003 [2]苏州大学附属儿童医院心内科,江苏苏州215003

出　　处：《苏州大学学报（医学版）》2008年第3期386-389,共4页Suzhou University Journal of Medical Science

基　　金：江苏省社会发展计划项目(No.BS2005023)

摘　　要：目的观察血管紧张素转换酶抑制剂(ACEI)对高肺血流量大鼠肺组织中血管紧张素Ⅱ(AngⅡ)的含量、血浆一氧化氮(NO)含量的影响。方法将23只雄性SD大鼠随机分为对照组(n=7)、分流组(n=8)和分流+依那普利组(n=8)。对分流组和分流+依那普利组大鼠行腹主动脉-下腔静脉分流术,并以依那普利进行干预。11周后以右心导管法测定肺动脉平均压(mPAP)、右心室/体质量(RV/BW)和右心室/(左心室+室间隔)比值[RV/(LV+S)],观测肺血管显微结构的变化,计算血管壁肌层厚度占外径的百分比(WT%)及血管壁肌层面积占血管总面积的百分比(WA%)。用放射免疫分析法测肺组织中AngⅡ的含量,并以分光光度计测定血浆NO含量。结果(1)分流组大鼠mPAP、RV/BW及RV/(LV+S)比值明显高于对照组;光镜下分流组大鼠肺小血管肌化程度明显增强,肺中、小型肌型动脉WT%和WA%明显升高;肺组织AngⅡ含量明显增加,血浆NO含量较对照组下降。上述差异均有统计学意义(均P<0.05)。(2)分流+依那普利组大鼠mPAP、RV/BW、RV/(LV+S)比值明显低于分流组,肺小血管肌化程度明显改善,中、小型肺肌型动脉WT%和WA%明显降低,肺组织AngⅡ含量有下降趋势,血浆NO含量较分流组明显增加。上述差异均有统计学意义(均P<0.05)。结论ACEI能减缓高肺血流量所致肺动脉高压和肺血管重塑,AngⅡ、NO可能在其中起重要作用。Objective To explore the therapeutic mechanism and effect of ACEI on pulmonary hypertension and pulmonary vascular structural remodeling induced by high pulmonary blood flow in rats. Methods Twenty-three male SD rats were randomly divided into the control group, shunting group and shunting with enalapril group. Abdominal aorta and inferior vena cava shunting were produced in rats of shunting group and shunting with enalapril group. After 11-week shunting, pulmonary artery mean pressure （mPAP） of each rat was measured by using a right cardiac catheterization procedure. The ratio of right ventricular mass to body weight （RV/BW） and the ratio of right ventricular mass to left ventricular plus septal mass [RV/（LV＋S）] were documented. Pulmonary vascular microstructure was measured. The ratio of vascular wall thickness to vascular external diameter （WT%） and the ratio of vascular wall area to total vascular area （WA%） were documented. Meanwhile, angiotensin Ⅱ （Ang Ⅱ） contents of lung tissue was measured by radioimmunoassay. The concentration of plasma nitric oxide （NO） was measured by spectrophotometry. Results （1） After 11-week aortocaval shunting, mPAP, RV/BW and RV/（LV＋S） were significantly increased in shunt rats as compared with those in the normal controls respectively. Muscularization of small pulmonary artery, WT% and WA% of pulmonary muscularized arteries were obviously increased in shunt rats compared with those in the controls. Meanwhile, in the lung tissue, Ang Ⅱcontents also increased significantly, plasma NO concentration was significantly decreased.（2）However, mPAP, RV/BW and RV/（LV＋S） were significantly decreased in rats in the shunting with enalapril group as compared with shunt rats. WT% and WA% of pulmonary muscularized arteries were obviously decreased in rats in the shunting with enalapril group compared with shunt rats. Meanwhile, in the lung tissue, Ang Ⅱ contents also decreased significantly, plasma NO concentration was significantly incr

关 键 词：肺动脉高压 血管紧张素转换酶抑制剂 血管紧张素Ⅱ 一氧化氮 

分 类 号：R972[医药卫生—药品]