^(99)Tc^m-EC-MN鼻咽癌乏氧显像的实验研究  

The Experimental Study of ^(99)Tc^m-EC-MN in Tumor Hypoxia Imaging

作　　者：金振军[1] 唐军[2] 杨仪[2] 刘增礼[2] 

机构地区：[1]苏州大学附属常熟医院放疗科,江苏常熟215500 [2]苏州大学附属第二医院核医学科,江苏苏州215004

出　　处：《苏州大学学报（医学版）》2008年第3期398-401,411,共5页Suzhou University Journal of Medical Science

摘　　要：目的通过对99Tcm-EC-MN在鼻咽癌体外及体内模型中生物学分布的研究,探讨99Tcm-EC-MN肿瘤乏氧显像的临床意义。方法利用99TcmO4标记EC-MN,纸层析法测定标记率。99Tcm-EC-MN分别加入正常条件和乏氧条件下培养的鼻咽癌细胞株内,在不同时间段检测99Tcm-EC-MN在细胞内的分布差别。建立鼻咽癌裸鼠肿瘤模型,自荷瘤鼠尾静脉注入99Tcm-EC-MN 3.7 MBq(0.1 ml)后0.5、1、2、4、6和8 h分别进行平面显像,观察99Tcm-EC-MN的体内分布情况。在各时相分别处死荷瘤裸鼠,取各脏器组织标本、称重并测量放射性计数,计算各标本每克组织百分注射剂量率(%ID/g)。对分离出的瘤体,应用CD34单克隆抗体及血管内皮生长因子(VEGF)单克隆抗体进行免疫组化染色。结果正常条件和乏氧条件下培养的鼻咽癌细胞株对99Tcm-EC-MN的吸收差异有高度统计学意义(P<0.01)。所有荷瘤裸鼠的肿瘤均呈阳性显像,静脉注射后0.5 h肿瘤灶即显影,4 h时显影最清晰。肿瘤/肌肉比值1、2、4 h分别达2.24倍、4.75倍、6.41倍。99Tcm-EC-MN在正常组织中清除快,主要经泌尿系统及肠道排出。免疫组化结果和放射性计数结果具有显著相关性(r>0.9,P<0.01)。结论99Tcm-EC-MN作为一种乏氧显像剂,具有良好的开发前景。Objective To study the biological distribution of ^99Tc^m-EC-MN in tumor models bearing nasopharyngeal carcinoma in vivo and that in vitro, and to explore the clinical significance of ^99Tc^m-EC-MN in tumor hypoxia imaging. Methods EC-MN was labeled with technetium- 99m, and the labeling efficacy was determined by paper chromatography. ^99Tc^m-EC-MN was added into the cell strains of nasopharyngeal, carcinoma under the normal and hypoxia condition respectively,difference of distribution was examined in different periods of time. Tumor nude mice models bearing nasopharyngeal carcinoma was developed. ^99Tc^m-EC-MN（3.7 MBq） was injected into caudal vein in eight nude mice, and planar scanning was performed to observe the concentration of the ^99Tc^m-EC-MN in tumors after 0.5,1,2,4,6,8 h. The tumor-bearing nude mice were sacrificed in different phases after injection, and radioactive counts in tissue samples from various organs were measured to calculate %ID/g. The immunohistochemical study of tumor tissue was observed by using CD34 McAb and VEGF McAb. Results Significant difference （P〈0.01） was detected in the absorption of ^99Tc^m-EC-MN between the cells of nasopharyngeal carcinoma under the normal conditions and those under the hypoxia condition. All of the tumor-bearing nude mice showed positive imaging, especially in 4 h after injection. The ratio between tumor and muscle in 1, 2, 4 h after injection was 2.24, 4.75 and 6.41. ^99Tc^m-EC-MN was rapidly removed from the normal tissue, mainly by the discharge from intestinal and urinary system. The results of immunohistochemical analysis was correlated with the radioactivity counts in tumor tissue （r〉0.9,P〈0.01）. Conclusion As a hypoxia imaging agent,^99Tc^m-EC-MN has good prospects of exploitation.

关 键 词：^99Tc^m-EC-MN 鼻咽癌 乏氧显像 生物学分布 免疫组化 

分 类 号：R817.4[医药卫生—影像医学与核医学]