高效液相色谱法测定野蔷薇果中V_C含量的研究  

DETECTION OF VITAMIN C IN MULTIFLORA FRUIT WITH HPLC

作　　者：迪丽努尔.马里克 沙拉买提[1] 曼苏尔[1] 

机构地区：[1]新疆师范大学生命科学与化学学院,新疆乌鲁木齐830054

出　　处：《食品研究与开发》2008年第7期87-89,共3页Food Research and Development

基　　金：国家自然科学基金(20465002)项目;自治区少数民族科技骨干人才特殊培养科技专项项目(2005-04)

摘　　要：建立一种高效液相色谱法测定野蔷薇中VC含量的新方法。采用Diamonsil5μ C18反相柱(200mm×4.6mm×5μm)分离;流动相为甲醇-0.01%磷酸水溶液(3:7,体积分数);柱温25℃;流速1.0mL/min;二级管陈列检测器,检测波长254nm。VC在浓度为20mg/L-1500mg/L范围内呈良好的线性关系,线性回归方程为y=17.136x+120.8,相关系数为0.9992,方法回收率为97%-101%相对标准偏差小于3%。该方法简便、快速、准确、适合于测定其它水果和蔬菜中VC的含量。A new method of detecting the conent of Vc in the muhiflora fruit by using HPLC is established.Methods ：The sepration was carried out on a Cls column （200 mm×4.6mm×5 μm）.Methanol-0.01H3PO4（3：7, V：V ） as mobile phase.The column temperature was set at 25 ~C.The flow rate at 1.0mL/min and the detection wave- length at 254 nm.Resuhs＇The standard working curve of Vc has a good linear relation in the, concentration range from 20 mg/L-1 500 mg/L, the linear equation is y=17.136 x＋ 120.8 and the related coefficient is 0.999 2.The recoveries as 97 %-101% and the relatively standard deviation is below 3 %.Conclusions：The method is simple and fast and exact and steady and suitable to detect the conent of Vc in other fruit and vegetable.

关 键 词：高效液相色谱 野蔷薇 VC 

分 类 号：R284[医药卫生—中药学]