谷氨酰胺肠外营养对大鼠移植小肠营养作用的研究  

Nutritional effects of glutamine enriched parenteral nutrition on transplanted small intestine in the rat

作　　者：张宏伟[1,2] 吴肇汉[1,2] 张延伟[1,2] 黄德骧 谢建新[1,2] 左焕琛[1,2] 

机构地区：[1]上海医科大学附属中山医院普外科 [2]上海医科大学解剖教研室

出　　处：《中华外科杂志》1997年第9期558-560,共3页Chinese Journal of Surgery

摘　　要：作者研究了加谷氨酰胺的肠外营养对大鼠移植小肠萎缩及功能低下的预防作用。对Ｗｉｓｔａｒ鼠行近段空肠移植，然后给予肠外营养１０天。实验组给予加３％丙氨酰－谷氨酰胺的营养液，而对照组给予含等氮量非必需氨基酸的营养液。结果显示：实验组移植小肠的绒毛高度、粘膜厚度、腺窝深度和绒毛表面积均明显大于对照组；实验组移植小肠粘膜上皮细胞的超微结构基本保持完好，而对照组则出现线粒体肿胀，嵴断裂和微绒毛萎缩。实验组移植小肠对１５Ｎ－甘氨酸的吸收率明显高于对照组。结果提示：加谷氨酰胺的肠外营养能促进大鼠移植小肠粘膜增生，维持粘膜细胞超微结构的完整，并能改善其对氨基酸的吸收能力。We evaluated the preventive effects of glutamine enriched pareneral nutrition on the atrophy and hypofunction of the transplanted small intestines in the rat.Wistar rats received jejunal isografts and central venous catheters for parenteral nutrition (PN) for ten days.Rats received either PN with 3% alanyl glutamine or the PN with isonitrogenous ballanced nonessential amino acids.Glutamine enriched PN significantly increased mucosal villous height mucosal thickness,crypt depth and villous surface area compared with the non glutamine PN.Normal enterocyte ultrastructure of the graft was maintained in the Glutamine enriched group.Atrophied microvilli and broken mitochondrial crista were observed in the control group.It was found that the amount of absorption of 15 N glycine was greater at the 1st,2nd,3rd hour in glutamine enriched group than that in the control.These results indicate that the glutamine enriched parenteral nutrition can promote the proliferation of mucosa,maintain the ultrastructure and improve the amino acid absorption in the transplanted small bowel in the rat.

关 键 词：谷氨酰胺 胃肠外营养 肠吸收 肠移植 

分 类 号：R656[医药卫生—外科学] R459.3[医药卫生—临床医学]