SOX10 mRNA在先天性巨结肠肠壁中的表达  

The expression of SOX10 mRNA in the Hirschsprung's disease Patient's bowel

作　　者：黄华[1] 陈琦[2] 曹振杰[2] 侯广军[1] 张二划[1] 黄敏[1] 杨启政[2] 

机构地区：[1]郑州市儿童医院外一科,河南省450053 [2]郑州大学第三附属医院儿外科,河南省450053

出　　处：《临床小儿外科杂志》2008年第3期30-32,共3页Journal of Clinical Pediatric Surgery

摘　　要：目的研究性别决定区Y基因相关高可变区基因10(SRY related high mobilitygroup-BoX gene10,SOX10)在先天性巨结肠(Hirschsprung’s disease,HD)肠壁中的表达情况,以进一步了解HD在分子基础上的发病机制。方法分别取12例先天性巨结肠病例的手术标本狭窄段、移行段及扩张段,随机取12例非巨结肠手术病例(如结肠造瘘或关瘘手术)作为对照组,提取平滑肌组织总RNA,应用逆转录多聚酶链反应(RT-PCR)扩增目的基因和看家基因片段,观察病变段和正常段SOX10mRNA的表达,并与看家基因(β-actin)在病变段和正常段的表达比较,进行统计学分析。结果SOX10mRNA在HD患儿痉挛段呈低表达,在扩张段及正常对照组呈高表达。痉挛段SOX10mRNA的表达量与移行段、扩张段及正常对照组比较,差异均有统计学意义;而移行段、扩张段及对照组比较,差异无统计学意义。结论HD患儿结肠SOX10mRNA的异常分布显示SOXIO基因是出生后肠神经系统维持正常功能所必需的,SOX10mRNA表达减少可引起肠管痉挛,肠腔狭窄,造成肠功能障碍。Objective To investigate the expression of SOX 10 mRNA in the sporadic Hirschsprung＇ s disease （HD） patient＇ s bowel to understand the molecular basis for the pathogenesis of HD. Methods Smooth muscle tissues from diseased and normal segments of 12 patients with HD were isolated respectively and preserved in -80~C refrigerator. RNA of each segment was isolated, then the DNA of SOXIO and a house keeper gene （β - actin） were amplified by using reverse transcription poly-merase chain reaction （RT-PCR） technique. The data of results were analyzed with the SPSS10.0 statistical software. Results Use paired-samples t test, the expression of SOX 10 mRNA in spastic segment was lower that of in transitional segment （p 〈 0.05） and normal segment （p 〈 0.05） in HD patients. While the expressions were not significantly different between transition segment and normal segment （P〉 0.05） in HD patients. Conclusions The differ SOX10mRNA expression in differ segments in non-syndromic HD patients and normal infants concluded that SOX 10 is normally required postnatally in the functional maintenance of the entire enteric nervous system, if the absence of ganglion cells expression reduced ,it may be aroused the intestine spastic and stricture and made its function obstacle.

关 键 词：Hirschsprung病/外科学 RNA 信使 

分 类 号：R726.5[医药卫生—儿科]