蛋白质溶液的膜结晶:膜结晶法结晶溶菌酶的研究  

Membrane crystallization of protein solutions:the study of lysozyme membrane crystallization

作　　者：魏可贵[1] 张新妙[1] 马润宇[1] 

机构地区：[1]北京化工大学生命科学与技术学院,北京100029

出　　处：《膜科学与技术》2008年第3期10-15,共6页Membrane Science and Technology

基　　金：国家自然科学基金资助项目(20676007)

摘　　要：用动态膜结晶法对溶菌酶结晶进行研究，考察了各种结晶条件对溶菌酶晶形和膜通量的影响，并对结晶条件进行优化，得到了质量较高的溶菌酶晶体．结果表明，当溶菌酶浓度为20-30mg／mL，结晶剂NaCl浓度在40-90g／L，洗脱剂MgCl2浓度在160～250g／L，pH在4．7～8．2范围内，均能得到高质量的溶菌酶晶体．The continuous osmotic membrane crystallization method was adopted to study on the crystallization of lysozyme. The influences of various crystallization conditions on the shape and quality of lysozyme crystals were investigated and some high quality crystals obtained after the crystallization conditions were optimized. The experiments show that high quality lysozyme crystals can be obtained in the following conditions：the concentration of lysozyme was 20--30 mg/mL, 40--90 g/L NaCl as the crystallizing agent, 160--250 g/L MgCl2 as the stripping solutions and the buffer at pH 4.7--8.2.

关 键 词：溶菌酶 膜结晶 结晶条件 

分 类 号：TQ028.8[化学工程] Q51[生物学—生物化学]