不同方法检测宿主动物体内鼠疫菌F1抗原的应用  

Assessment of different methods for the detection of fraction 1 antigen of Yersinia pestis among animal hosts in plague surveillance program

作　　者：黄德蕙[1] 梁江明[1] 秦石英[1] 曾竣[1] 廖驰真[1] 鲁翠芳[1] 韦锦平[1] 陈达宗[1] 李寿生[1] 周树武[1] 农智[2] 罗寿军 林蓉 李红军[4] 覃刚[4] 农雪飞 热娜[5] 徐兵[5] 海荣[6] 

机构地区：[1]广西壮族自治区疾病预防控制中心,南宁530028 [2]广西百色市疾病预防控制中心,533000 [3]广西隆林县疾病预防控制中心,533400 [4]广西西林县疾病预防控制中心,533300 [5]新疆维吾尔自治区疾病预防控制中心,乌鲁木齐830002 [6]中国疾病预防控制中心传染病预防控制所,北京102206

出　　处：《应用预防医学》2008年第3期131-133,共3页Applied Preventive Medicine

基　　金：国家"十五"科技攻关项目;2004B718B07-01

摘　　要：目的了解不同方法在鼠疫监测现场中的应用效果,掌握检测方法的可行性、可操作性及对鼠疫监测的影响因素,评价方法间的一致性,制订适于现场应用的有效监测机制。方法对广西隆林、西林县鼠疫监测点收集的鼠脏器同时进行分离培养、反向间接血凝试验(RIHA)、胶体金免疫层析试验(RGICA)、酶联免疫吸附试验(ELISA)、聚合酶链反应(F1-Pla-PCR)。结果分离培养、RIHA、RGICA、ELISA、PCR检测鼠脏器中鼠疫菌F1抗原均为阴性,5种方法间一致性比较Kappa值等于0,呈轻度一致。结论新技术胶体金免疫层析试验、酶联免疫吸附试验、聚合酶链反应具有操作简便易学、特异、快速、敏感等特点,适宜在鼠疫监测现场中应用。Objective To evaluate the effectiveness, feasibility and operability of different methods for plague surveillance, in order to provide evidence for making appropriate control strategies. Methods Rat organ samples were collected from plague surveillance sites in Longan and Xilin counties. They were tested using culture, RIHA,GICA （gold immunochromatographic assay）, ELISA and F1 - Pla- PCR. Results All the samples were negative for F 1 antigen with the 5 methods. The Kappa value was 0, indicating the agreement of the 5 tests was good. Conclusion RCICA, ELISA and PCR are easy to perform, specific, rapid and sensitive; therefore they are useful techniques in plague surveillance program.

关 键 词：鼠疫 宿主动物 F1Ag 检测 现场应用 

分 类 号：R516.84[医药卫生—内科学]