HPLC法测定补肾健脑软胶囊中补骨脂素和异补骨脂素的含量  

Determination of Psoralen and Isopsoralen in Bushen Jiannao Soft Capsule by HPLC

作　　者：徐晓明 徐大勇 邢俊波[3] 

机构地区：[1]梅河口市卫生职工中等专业学校,吉林梅河口135000 [2]梅河口市医院爱民医院药剂科,吉林梅河口135000 [3]中国人民解放军总后卫生部药品仪器检验所,北京100071

出　　处：《中国中医药信息杂志》2008年第7期47-48,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立补肾健脑软胶囊中补骨脂素和异补骨脂素的含量测定方法。方法采用HPLC法,色谱柱为Diamonsil-C18(250mm×4.6mm,5μm),流动相为甲醇-水(48∶52),流速为1.0mL/min,检测波长为246nm,柱温40℃。结果补骨脂素和异补骨脂素线性范围分别为0.01008～0.252μg(r=0.9998)、0.00914～0.2285μg(r=0.9999)。平均回收率分别为98.91%(RSD=1.74%)、99.93%(RSD=1.27%)。结论本法分离度好,快速、简便,可作为该产品的质量控制方法。Objective To establish a method for determination of psoralen and isopsoralen in Bushen Jiannao Soft Capsule. Methods The HPLC separation was performed on Diamonsil-C18 （250 mm×4.6 mm, 5 μm） column with a mixture methanol-water （48 ： 52） as the mobile phase, at a flow rate of 1.0 mL/min, Detection wavelength was at 246 nm, and column temperature at 40 ℃. Results The Linear response of psoralen and isopsoralen ranged from 0.010 08-0.252 μg （r=0.999 8） and 0.009 14-0.228 5 μg （r= 0.999 9）. The average recovery was 98.91% （RSD=1.74%） and 99.93% （RSD=1.27%） respectively. Conclusion The method is simple, accurate, and suitable for the determination of the psoralen and isopsoralen in the preparations.

关 键 词：补肾健脑软胶囊 补骨脂素 异补骨脂素 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]