小卷蛾斯氏线虫β-微管蛋白基因的克隆与序列分析  

Cloning and Sequences analysis of β-Tubulin cDNA from Steinernema carpocapsae

作　　者：秦松柏[1] 樊东[2] 李春杰[3] 许艳丽[3] 

机构地区：[1]东北林业大学,哈尔滨150040 [2]东北农业大学 [3]中国科学院东北地理与农业生态研究所

出　　处：《东北林业大学学报》2008年第7期72-74,83,共4页Journal of Northeast Forestry University

基　　金：中国科学院知识创新工程重要方向项目(KZCX2-YW-408)

摘　　要：用TRIZOL法提取了小卷蛾斯氏线虫RNA，利用RT-PCR和cDNA末端快速扩增技术（RACE）技术克隆了两条小卷蛾斯氏线虫β-微管蛋白基因cDNA的3＇端序列。将得到的cDNA序列提交到GenBank，分别获取登录号EU049857和EU049858。两个序列，长度分别为1372碱基（SCTUBl）和1374碱基（SCTUB2），分别编码434和432个氨基酸，编码的蛋白的分子量在48kDa左右。氨基酸的124-131位存在一个微管蛋白信号片段GGGTGSG。序列分析表明，分离得到的两个基因与其他线虫的微管蛋白基因具有较高的同源性，核苷酸序列同源性在65％以上，氨基酸序列同源性在81％以上，两个氨基酸序列都含有两个氨基酸保守区NNWAKGHY和RKAFLHWYTGEGMDEMEFTE。The Total RNA was isolated from Steinernema carpocapsae. Two β-tubulin sequences of S. carpocapsaeWere cloned by RT-PCR and rapid amplification of cDNA ends （RAGE） The two β-tubulin sequences were designated as SCTUB1 and SCTUB2 and submitted to Genbank under the accession numbers EU049857 and EU049858. SCTUB1 and SCTUB2 respectively contained 1 372 base pairs and 1 374 base pairs with deduced amino acid sequences of 434 residues and 432 residues. The predicted molecular weight of these two sequences was about 48 kDa. There was a tubulin signature sequence GGGTGSG at 124-131 sites of the deduced amino acid. The sequence analysis indicated that the isolated nucleotide fragments and the deduced amino acid sequences both shared high identity with the sequences from other nematodes. The identity of nucleotides and amino acid residues were 65% and 81% respectively. The deduced amino acid sequences shared two conserved amino acid sequence motifs NNWAKGHY and RKAFLHWYTGEGMDEMEFTE with other nematode tubulins.

关 键 词：小卷蛾斯氏线虫(Steinernema carpocapsae) Β-微管蛋白 克隆 序列分析 

分 类 号：S154.386[农业科学—土壤学]