溴隐亭对植物血凝素诱导活化T淋巴细胞的影响  

Effect of Bromocriptine on the Activation of T Lymphocyte Stimulated by Phytohemagglutinin

作　　者：石瑛[1] 毛兰芝[2] 余保瑞[3] 胡志军[1] 张婧婧[1] 高志涛[2] 王辉[1] 

机构地区：[1]新乡医学院医学检验系,河南新乡453003 [2]新乡医学院免疫学研究中心,河南新乡453003 [3]河南省人民医院心内科,郑州450003

出　　处：《实用儿科临床杂志》2008年第11期857-859,共3页Journal of Applied Clinical Pediatrics

基　　金：河南省科技攻关项目资助(611042700)

摘　　要：目的研究溴隐亭(BRC)对植物血凝素(PHA)诱导活化T淋巴细胞的影响。方法用泌乳素(PRL)与BRC单独和联合刺激培养PHA诱导活化的CD4+T淋巴细胞系JurkatE6-1细胞,利用实时聚合酶链反应(RT-PCR)技术检测活化T淋巴细胞重要信号分子转接蛋白(LAT)、T细胞受体相关的相对分子质量为70000的蛋白激酶ζ链(ZAP-70)mRNA表达变化情况;利用Realtime-PCR方法检测活化T淋巴细胞自身PRLmRNA表达变化情况;利用流式细胞术检测T淋巴细胞活化早期表面标志细胞分化抗原CD25分子表达变化情况;利用脂质体转染荧光素酶报告系统检测T淋巴细胞活化的核转录因子-κB(NF-κB)活性变化情况。结果1.BRC可抑制PHA诱导的T淋巴细胞活化和PRL-泌乳素受体(PRLR)信号转导途径的共同信号分子ZAP-70mRNA及活化T淋巴细胞自身PRLmRNA的表达;2.BRC促进了活化T淋巴细胞信号分子LATmRNA和活化T淋巴细胞表面CD25分子的表达;3.BRC抑制了PHA诱导的活化T淋巴细胞核NF-κB活性。结论BRC可通过抑制T淋巴细胞活化和PRL-PRLR途径中的共同信号分子ZAP-70及活化T淋巴细胞自身PRLmRNA分泌,对T淋巴细胞活化发挥抑制作用。Objective To study the effect of bromoeriptine （BRC） on the activation of T lymphocyte stimulated by phytohemagglutinin （PHA）. Methods After CD4^ ＋ T cell line Jurkat E6 - 1 cells were stimulated by PHA, prolaetin （PRL） and BRC, respectively, the expression of linker for activation of T cells （LAT） and zeta - chain T cell receptor associated protein kinase 70 000 （ ZAP - 70 ） mRNA of T lymphoeytes were checked by RT - PCR. The expression of PRL mRNA of T lymphoeytes was detected by Real time PCR. The expression of CD25 （ cluster of differentiation） as a marker of early activation on the surface of T lymphoeytes was detected by flow eytometry, and the activation of nuclear factor - κB （NF - κB） was detected by lueiferase reporter system. Results 1. BRC inhibited the expression of ZAP -70 as the common signal molecules both in the T lymphocyte activation pathway and PRL - prolaetin - prolaetin receptor（PRLR） signal transduetion pathway, and decreased the expression of PRL mRNA produced by activation T lymphoeytes. 2. BRC enhanced the expression of LAT mRNA as another important signal molecular on the T lymphoeytes and CD25 on the surface of the T lymphoeytes. 3. The activation of NF - κB of T lymphoeytes was decreased. Conclusions BRC might inhibit the activation of T lymphoeytes by inhibiting the expression of ZAP - 70, the common signal molecules between T lymphoeytes activation and PRL - PRL pathway, and PRL mRNA, the like - T lymphocyte growth factor.

关 键 词：溴隐亭 ZAP-70 活化T淋巴细胞重要信号分子转接蛋白 泌乳素 核因子-ΚB 

分 类 号：R96[医药卫生—药理学]