HPLC法测定交沙霉素片中交沙霉素的含量  

Determination of content of josamycin in josamycin tablets by HPLC

作　　者：常一丁[1] 

机构地区：[1]北华大学附属医院,吉林吉林132001

出　　处：《中国热带医学》2008年第7期1239-1240,共2页China Tropical Medicine

摘　　要：目的用HPLC法测定交沙霉素的含量。方法以交沙霉素标准品作对照,采用HPLC法测定交沙霉素片中的交沙霉素含量。色谱柱:CAPCELL PAK C8;TYPE:DD5μm;SIZE:4.6mm.I.D.×150?;检测波长:231nm;流动相:乙酸铵(0.01mol/L)、甲醇:乙腈(55:5),采用外标法。结果在优化色谱条件下,交沙霉素线性范围10～80μg,r=0.99995,回归方程为:A=7.4872C+1551.2522。含量测定的回收率为:97.24%RSD=1.22%。结论该方法专属性强,操作简便,结果准确,重现性好,适用于对交沙霉素片中交沙霉素含量的测定。Objective To established a high performance liquid chromatography for determination of josamycin. Methods Standard article of josamycin was used as control and the content of josamycin was determined with HPL on a column of CAPCELL PAK C8;TYPE：DD 5μ and a size of 4.6mm. I.D. × 150mm;the ultraviolet detection was 210nm;equilibrated with an eluent mixture and ammonium acete methand - aeetonitrle Results The linear range was 10 - 80μg ,r = 0. 99995 and the recovery was 97.24% ,the RSD was 1.22%, Conclusion The method is special , accurate, simple, reproducible for determination of josamycin in the Josamycin tablets.

关 键 词：高效液相色谱法 交沙霉素 片剂 含量测定 

分 类 号：R978.1[医药卫生—药品]