L-肌肽对高热惊厥幼鼠神经细胞凋亡的影响  

Effect of L-Carnosine on Neuronal Cell Apoptosis in Young Rats with Febrile Seizures

作　　者：许建文[1] 张光军[2] 邱培勇[2] 陈正跃[2] 王家勤[1] 郭学鹏[1] 

机构地区：[1]新乡医学院第三附属医院儿科,河南新乡453003 [2]新乡医学院药物研究室,河南新乡453003

出　　处：《实用儿科临床杂志》2008年第12期913-914,967,共3页Journal of Applied Clinical Pediatrics

基　　金：河南省医药卫生科技攻关项目资助(200698)

摘　　要：目的探讨L-肌肽对幼年大鼠实验性高热惊厥后神经细胞凋亡的影响。方法40只15日龄SD大鼠采用随机数字表法分为L-肌肽干预组(n=30)和惊厥对照组(n=10)。采用热水浴法诱导干预组和惊厥对照组大鼠实验性FS发作10次;干预组又随机分为E、G、H组,分别在每次惊厥发作后30、60、120min腹腔注射L-肌肽250mg/kg;惊厥对照组仅诱发癫,不给予干预。末次惊厥后12h处死大鼠,并采用原位末端脱氧核苷酸生物素标记法分别测定幼鼠海马结构及大脑皮层神经元凋亡情况,透射电镜观察其凋亡细胞超微形态学改变。结果惊厥对照组神经细胞凋亡计数为25.37±1.95,E组为12.36±1.13,G组为17.85±2.04,H组为22.69±2.69。惊厥对照组神经细胞凋亡明显高于干预组(F=10.75P<0.01);给予L-肌肽腹腔注射后E组和G组神经细胞凋亡明显低于惊厥对照组(t=18.26,8.45Pa<0.01);但H组神经细胞凋亡与惊厥对照组无明显差异(t=2.55P>0.05)。透射电镜观察可见,惊厥对照组和H组大鼠海马CA1区、齿状回神经元损伤明显高于E组。结论早期给予L-肌肽具有减轻反复FS引发的幼鼠神经细胞凋亡,具有一定的保护神经细胞作用。Objective To explore the effect of L-carnosine on neuronal cell apoptosis in young rats with experimental febrile seizures （FS）. Methods Forty 15-day SD rats were randomly divided into intervention group （n = 30 ） and FS group （n = 10）. Warm water was used to induce 10 times FS. The intervention group was divided into E, G and H group, 10 rats in each group. Iintraperitoneal injection of L-carnosine（250 mg/kg）was separately given to the rats in E group, G group and H group respectively after 30, 60 and 120 min of seizure. FS group were induced FS, but they were not given intervention. The rats were sacrificed at 12 hours after the last seizure. Neuronal cell apoptosis was determined by terminal eoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） in situ cell death kit. TUNEL positive cells were stained and counted as apoptosis in hippocampus and cortex. Ultrastructural changes of apoptosis neurons were observed under the electron microscope. Results The neuronal cells apoptosis count was 25.37±1.95 in FS group, 12.36±1.13 in E group, 17.85±2.04 in G group, and 22.69±2.69 in H group. Neuronal apoptosis of FS group was apparently higher than that of interventional groups （ F= 10.75 P 〈 0.01 ）. Neuronal apoptosis of E group and G group were obviously lower than FS group after intraperitoneal injection of L-canrosine （t = 18.26,8. 45 Pa〈 0.01 ）. Neuronal apoptosis of H group was not obviously lower than that in FS control group （t= 2. 55 P〉0. 05 ）. Under the electron microscope, neuronal damage on hippocampal CA1 area and dentate gyrus of FS group and H group was obviously higher than that of E group. Conclusions Early injection of L-carnosine would not only relieve neuronal apoptosis of repeated FS, but also play a role in the protection of neuronal cells.

关 键 词：L-肌肽 高热惊厥 神经细胞 凋亡 

分 类 号：R720.597[医药卫生—急诊医学]