胆红素脑病仔鼠脑组织神经元特异性烯醇化酶mRNA及其蛋白的动态变化  

Dynamic Changes of Neuron-Specific Enolase mRNA and Protein in Brain Tissue of Offspring Rats with Bilirubin Encephalopathy

作　　者：姜志梅[1] 李晓捷[1] 孙爱萍[2] 陈炳坤[3] 

机构地区：[1]佳木斯大学附属第三医院发育与行为儿科 [2]北京同仁医院康复中心,北京100176 [3]佳木斯大学康复医学院,佳木斯154003

出　　处：《实用儿科临床杂志》2008年第12期915-917,922,共4页Journal of Applied Clinical Pediatrics

基　　金：黑龙江省教育厅科学技术研究项目资助(10551313);佳木斯大学科研项目资助(s2006-25)

摘　　要：目的观察胆红素脑病仔鼠脑组织神经元特异性烯醇化酶(NSE)mRNA及其蛋白的变化,探讨其生物学机制和对胆红素脑病的诊断价值。方法选取新生7日龄清洁级Wistar大鼠120只,随机分为对照组和实验组,各60只,每组再按6个时间点(6、12、24、48、72、96h)分为6组,每组10只。对照组仔鼠腹腔注射9g/L盐水0.5mL,实验组仔鼠腹腔注射胆红素溶液200mg/kg制成胆红素脑病模型。应用反转录-聚合酶链反应技术检测胆红素脑病仔鼠不同时间点脑组织NSEmRNA表达;应用免疫组织化学方法检测不同时间点海马区、皮质区、丘脑区及苍白球区脑组织NSE蛋白阳性表达。结果实验组大鼠脑组织NSEmRNA表达自6h开始明显下降,与对照组比较均有显著性差异。造模后6h其海马区脑组织NSE蛋白阳性表达下降,实验各组与对照组比较无显著性差异;其皮质区脑组织NSE蛋白阳性表达6～96h均明显下降,与对照组比较均有显著性差异;造模后6h其丘脑区脑组织NSE蛋白阳性表达开始下降,实验组6、12、48、96h与其对照组比较均无显著性差异,而24、72h与其对照组比较有显著性差异;苍白球区脑组织NSE蛋白阳性表达6～96h明显下降,与对照组比较均有显著性差异。结论NSEmRNA的变化同其蛋白的变化趋势一致,是判定胆红素脑病的可靠神经生化指标之一。Objective To observe the dynamic changes of neuron-specific enolase （NSE） mRNA and protein in offspring rats brain tissue with bilirubin encephalopathy and explore the pathological mechanism and its diagnostic value on bilirubin encephalopathy. Methods Seven-day postnatal Wistar rats were used for study. One hundred and twenty rats were divided into 2 groups randomly （ control group and experimental group）, which were respectively subdivided into 6 groups （6, 12, 24, 48,72, 96 h）. The rats in control group were intraperitoneally administered physiological saline 0.5 mL, the rats in experimental groups were intraperitoneally administered bilirubin （200 mg/kg）. Reverse transcription-polymerase chain reaction was used to analyze the dynamic changes of NSE mRNA expression at 6, 12, 24, 48, 72 and 96 h in brain tissue of rats with bilirubin encephalopathy. Immunohistochemistry was used to evaluate NSE protein expression in hippocampi, cerebral cortex, thalamic and pallidus at different times. Results The expression of NSE mRNA significantly decreased in brain tissue of rats with bilirubin encephalopathy from 6 h to 96 h compared with the control groups. The expression of NSE protein in hippocampi decreased in offspring rats with bilirubin eneephalopathy from 6 h to 96 h, but there were no differences compared with the control groups. The expression of NSE protein in cerebral cortex was significantly decreased in rats with bilirubin encephalopathy from 6 h to 96 h, there were significant differences compared with the control group. The expression of NSE protein in thalamic significantly decreased in rats with bilirubin eneephalopathy from 6 h to 96 h, but there were significant differences between experimental groups and the control groups at 24 h and 72 h. The expression of NSE protein in pallidus significantly decreased in offspring rats with bilirubin encephalopathy from 6 h to 96 h, and there were significant differences compared with control groups. Conclusions The changing trends of expression of

关 键 词：大鼠 胆红素脑病 神经元特异性烯醇化酶 信使核糖核酸 

分 类 号：R722.1[医药卫生—儿科]