高效液相色谱法测定叶下珠中叶下珠素含量  

HPLC determination of phyllanthin constituent in Phyllanthus urinari L

作　　者：刘高[1] 饶力群[1] 范适[2] 杨华[1] 彭国平[1] 李兰岚[1] 龙晓蕾[1] 陈曦[1] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]湖南环境生物职业技术学院园林系,湖南衡阳421005

出　　处：《湖南农业大学学报（自然科学版）》2008年第3期277-280,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省科技计划项目(06SK3061);湖南省教育厅青年基金项目(04B018)

摘　　要：建立高效液相色谱测定不同产地叶下珠中叶下珠素含量的方法．采用色谱柱SinoChrom ODS柱，通过考察HPLC的检测波长和流动相，确定检测波长为230nm，流动相MeOH—H2O（体积比80：20），等梯度洗脱，流速0．8mL／min．结果表明，叶下珠素在2．72～136μg／mL时，叶下珠素质量浓度与峰面积呈良好的线性关系，回归方程为y=27．284x＋19．804（r=-0．9991），平均回收率可达98．26％．HPLC method for determination of content of phyllanthin in Phyllanthus urinaria L. plant samples was collected from different resourses. The HPLC of UV detection and gradient elution was reviewed. Confirmed separation was carded out on an ODS column and mixture of methanol-water（80 ： 20） as the mobile phase was used. The flowing rate was 0.8 mL/min and detection was affected at 230 nm. The experimental results showed that the assay displayed good linearity over the concentration range of 2.72- 136 μg/mL and the average recoverie was 98.26%.

关 键 词：叶下珠 叶下珠素 高效液相色谱法 

分 类 号：Q949.753.5[生物学—植物学]