反相高效液相色谱法同时测定车前子中的毛蕊花苷和异毛蕊花苷  

Determination of Verbascoside and Isoverbascoside in Semen plantaginis by RP-HPLC

作　　者：万茵[1] 谢明勇[1] 何彦林[1] 

机构地区：[1]南昌大学食品科学教育部重点实验室,南昌330047

出　　处：《天然产物研究与开发》2008年第3期474-476,共3页Natural Product Research and Development

基　　金：国家自然科学基金项目(30660226);长江学者和创新团队发展计划项目(IRT0540);2004年江西省科技攻关项目

摘　　要：采用反相高效液相色谱法在Waters Symmitry C18 （5 μm，250 mm×4.6 mm）色谱柱上以甲醇：体积分数0.1%甲酸溶液（40：60，v：v）为流动相同时测定车前子中毛蕊花苷和异毛蕊花苷的含量，流动相流速为0.6 mL/min，检测波长为330 nm。毛蕊花苷线性范围为0.2～2.0 μg，相关系数R＝0.9982。异毛蕊花苷线性范围为0.2～2.0 μg，R＝0.9957。样品的平均回收率分别为99.70 %，101.18%。测得大粒车前子中毛蕊花苷含量为8.65 mg/g，异毛蕊花苷含量为1.36 mg/g。此方法准确、快速，适用于车前子中毛蕊花苷和异毛蕊花苷的定量分析。A method for determining verbascoside and isoverbascoside in Semen plantaginis by RP-HPLC was described. The determination was carried out on a Waters Syrnmitry C18 （5 μm, 250 mm × 4.6 mm） column at 25 ℃. 0.1% HCOOH：MeOH （60：40, v：v） was used as mobile phase at a flow rate of 0.6 mL/min and detection wavelength was set at 330 ran. The linearity of verbascoside was found in the range of 0.2-2.0 μg,r = 0. 9982 ,the average recovery rate was 99.70%. The linearity of isoverbascoside was found in the range of 0.2-2.0 μg,r = 0. 9957, and the average recovery rate was 101.18%. The contents of verbascoside and isoverbascoside in Semen plantaginis were determined as 8. 65 mg/ g and 1.36 mg/g,respectively. This method is reliable,accurate and suitable for the determination of verbascoside and isoverbascoside in Semen plantaginis.

关 键 词：车前子 毛蕊花苷 异毛蕊花苷 反相高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] Q946.91[医药卫生—中医学]