激素对银杏愈伤组织诱导及继代培养的影响  

Effects of Hormone on Callus Induction and Subculture for Ginkgo biloba

作　　者：朱红威[1] 邵菊芳[1] 陶秀祥[1] 李庆[1] 王丹[1] 

机构地区：[1]中国矿业大学化工学院,徐州221008

出　　处：《天然产物研究与开发》2008年第3期482-487,共6页Natural Product Research and Development

基　　金：中国矿业大学科技基金项目(OH060101;OH061009)

摘　　要：为了探讨激素对银杏愈伤组织诱导及继代培养的影响,以银杏优良品种"佛手"无菌苗的叶片和根段为材料,以MS为基本培养基,研究了5种不同激素配比和光照条件对愈伤组织诱导及长期继代培养的影响。结果表明,所有激素配比都能诱导两种外植体形成愈伤组织,完全黑暗培养条件不利于愈伤组织继代培养;NAA和KT及2,4-D和KT两种激素组合分别有利于叶片和根愈伤组织诱导。在激素配比为3.0mg/L2,4-D+5.0mg/LKT中生长的两种愈伤组织鲜重显著高于其它处理;NAA和KT组合适合愈伤组织长期继代保存,叶片诱导的愈伤组织最佳继代培养基为NAA1.0mg/L+KT1.0mg/L,根段诱导的愈伤组织则为NAA1.0mg/L+KT1.0mg/L,分别在这两种激素组合下继代的愈伤组织始终为绿色,叶绿素含量变化不显著。Effects of hormone on callus induction and subculture were studied. Leaf and root of plantlets from species of ＂fushou＂ were used to establish callus cultures using MS medium with various hormone regimes：2,4-D （3.0 mg/L） with KT （ 1.0 and 5.0 mg/L） ;NAA （1.0 mg/L） with KT （0.5 and 1.0 mg/L） ;NAA （3.0 mg/L） with KT （1.0 mg/L）. All in vitro cultures were incubated under t6 h photoperiod or dark and temperature （25 ± 1 ） ℃. Callus have been obtained from all treatments using two kind explants, but their growth was severely limited under dark conditions when subcultured. Medium supplemented with 3.0 mg/L 2,4-D and 5.0 mg/L KT produced the overall highest weight for both leaf-derived and root-derived callus production. Chlorophyll content was used as an indicator to evaluate and confirm the best medium for callus production and long-term maintenance. Callus in medium supplemented with NAA and KT yielded less variation in color and appearance of callus. Medium with 1.0 mg/L NAA ＋ 1.0 mg/L KT and 1.0 mg/L NAA ＋ 0.5 mg/L KT were selected for callus production and long-term maintenance for leaf-derived and root-derived callus, respectively.

关 键 词：银杏 愈伤组织 继代培养 叶绿素含量 

分 类 号：Q943.1[生物学—植物学] R931[医药卫生—生药学]