荧光猝灭法测定痕量NO_2^-  

A fluorescence quenching method for determination of trace nitrite

作　　者：李芳[1] 孙向英[1] 

机构地区：[1]华侨大学材料学院,泉州362021

出　　处：《分析试验室》2008年第7期46-48,共3页Chinese Journal of Analysis Laboratory

基　　金：福建省自然科学基金(D0410019);福建省国际合作重点(2006I0021)项目资助

摘　　要：在HCl介质中,NO2-与5-氨基-1,2,3,4-四氢-1,4酞嗪二酮(ATPD)发生亚硝化反应,使ATPD的荧光猝灭,且其荧光猝灭程度与NO2-量呈线性关系,从而建立了测定痕量NO2-的新方法。该法线性范围为0.067~1.172μg/mL,方法检出限为0.70μg/L。本法已用于雨水中痕量NO2-的测定。A simple and sensitive fluorescence quenching method for the determination of trace nitrite was developed. The method is based on the diazo-reaction between nitrite ion and 5-Amino-1, 2, 3, 4-tetrahydro-phthalazin-1,4-dion （ATPD） in hydrochloric acid medium. The fluorescence intensity was measured with excitation and emission wavelengths of 365 and 428 nm, respectively. The linear range for the determination of NO2 - was 0.067 - 1. 172 μg/mL. The detection limit was 0.70 μg/L. The method was applied to the determination of trace nitrite in rain water samples.

关 键 词：NO2ˉ 5-氨基-1 2 3 4-四氢-1 4酞嗪二酮 荧光猝灭法 

分 类 号：O657.3[理学—分析化学]