罗丹明6G荧光猝灭法测定中草药中微量铁  

Determination of iron in chinese herbal medicine based on fluorescence quenching of rhodamine 6G

作　　者：王益林[1] 陆建平[1] 汤艳荣[1] 彭剑[1] 

机构地区：[1]广西大学化学化工学院,南宁530004

出　　处：《分析试验室》2008年第7期109-111,共3页Chinese Journal of Analysis Laboratory

基　　金：广西大学科研基金(校基金项目编号:X051108)资助项目

摘　　要：在H2SO4溶液中，Fe^3＋与过量KI反应后生成I3ˉ阴离子，I3ˉ与罗丹明6G形成离子缔合物，并使罗丹明6G发生荧光猝灭，据此建立了测定Fe^3＋的荧光猝灭分析方法。方法的线性范围为20～200μg/L，检出限为8．3μg/L，用于中草药中铁的测定。A fluorescence quenching method was developed for the determination of iron（Ⅲ ） with rhodamine 6G as a fluorescent reagent, Iron（Ⅲ） reacted with potassi.um iodide in dilute sulfuric acid solution to produce I3- anion which can react with rhodamine 6G to form a non-fluorescence compound to quench the fluorescence intensity of the system. The fluorescence intensity decreased linearly with the iron（Ⅲ ） concentration in the range of 20 to 200μg/L. The detection limit was 8.3 μg/L. It has been applied to the determination of iron（Ⅲ ） in the Chinese herbal medicine with satisfactory results.

关 键 词：罗丹明6G 荧光猝灭 FE^3+ 离子 

分 类 号：O657.31[理学—分析化学]