儿童原发性肾病综合征载脂蛋白B基因Msp I位点多态性研究  

STUDY ON MSP I LOCUS POLYMORPHISM OF APOLIPOPROTEIN B GENE IN CHILDREN UNDERGOING PRIMARY NEPHRITIC SYNDROME

作　　者：胡鹏[1] 覃远汉[1] 经承学[1] 雷凤英[1] 李铭芳[1] 

机构地区：[1]广西医科大学第一附属医院儿科,广西530021

出　　处：《现代预防医学》2008年第13期2533-2536,共4页Modern Preventive Medicine

基　　金：广西科学基金资助项目(桂科基0236029)

摘　　要：[目的]探讨载脂蛋白B(apoB)基因MspI位点多态性与儿童原发性肾病综合征(PNS)血脂代谢紊乱的关系。[方法]运用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术结合测序的方法分析150例PNS患儿和100例健康儿童ApoB基因MspI位点多态性,平衡法计算出各等位基因频率;检测肾病组血浆脂蛋白a(LPa)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、载脂蛋白A1(ApoA1)及ApoB水平。[结果](1)对照组ApoB基因MspI位点各基因型分布符合Hardy-Weinberg平衡,且与国内外研究比较未见显著性差异(P=0.68);(2)肾病组和对照组MspⅠ位点各基因型及等位基因频率分布未见显著差异(P﹥0.05);(3)PNS患儿ApoB基因MspI位点不同等位基因间各项血脂指标均未见明显差异(P﹥0.05)。[结论]尚不能认为ApoB基因MspI位点多态性对儿童PNS血脂代谢构成影响。[Objective] To probe the association between Msp I locus polymorphism of apohpoprotein B （apoB） gene and dyslipidemia in children undergoing primal- rephritic syndrome （PNS）. [Methods] Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） technique and gene sequence determination were used to analyze the Msp I genotypes of apoB gene among 150 PNS and 100 healthy children, alleles frequency were calctdated by balancing method; serum levels of lipoprotein a （LPa）, total cholesterol （TC）, triglyceride （TG）, high-density lipoprotein cholesterol （HDL-C） , low-density lipoprotein cholesterol （LDL-C）, ApoAl and ApoB were detected. [Results] （1） The distributions of the Msp I genotypes in healthy children were identical with Hardy-Weinberg equilibrium, and no signifieanees were observed in our study and the others at home and ahroad （P = 0.68） ; （2） the distributions of the Msp I genotypes and alleles frequency were no significant differences between cause group and control group （P 〉 0.05 ）; （3） there were no obvious disparities of serum lipids between different alleles of Msp I locus in PNS children （P 〉 0.05）. [ Conclusion] We can not draw a conclusion that lipids abnormalities is influenced by the Msp I locus polymorphism of apoB gene in children undergoing PNS.

关 键 词：载脂蛋白 多态性 等位基因 肾病综合征 总胆固醇 

分 类 号：R726.9[医药卫生—儿科]