诱导幼羔卵泡发育及体外胚胎生产  

Inducement of Lamb Follicular Development and Embryo Production In vitro

作　　者：陈晓勇[1] 田树军[1] 桑润滋[1] 孙树春[1] 赵驻军[1] 李俊杰[1] 敦伟涛[2] 

机构地区：[1]河北农业大学动物科技学院,保定071001 [2]河北省畜牧兽医研究所,保定071001

出　　处：《农业生物技术学报》2008年第3期456-460,共5页Journal of Agricultural Biotechnology

基　　金：国家科技支撑计划(No.2006BAD14B08-09);河北肉用绵羊品种选育项目(No.06220402D-3)资助

摘　　要：研究了羔羊年龄、运输应激和重复超排对幼羔所获卵母细胞数量的影响,以及胚胎发育液中添加乙二胺四乙酸(EDTA)对幼羔体外胚胎发育能力的影响。结果表明:6～8周龄组幼羔超排的每只平均获卵数和可用卵数(60.8枚和58.2枚)显著高于12～14周龄组(27.3枚和26.0枚)(P<0.05);运输应激不影响幼羔超排所获卵母细胞数量(P>0.05),但重复幼羔超排显著降低所获卵母细胞数量(P<0.05)。在胚胎发育液中加入10μmol/LEDTA可显著地提高幼羔体外早期胚胎的发育能力(P<0.05),并且部分体外胚胎移植后成功产下健康后代。Effect of lamb age, transport stimulation and repeated hormone superovulation on the number of collected oocytes were determined, and effect of the culture medium containing ethylene-diamine-tetra-acetic acid （EDTA） on the development of produced embryos from lamb oocytes in vitro were studied. Results indicated that the mean number of collected and available oocytes from per 6- to 8- week lamb were 60.8±13.9 and 58.2±12.3 respectively, which were more than that those （27.3±5.1 and 26.0±4.9 ） from per 12- to 14- week lamb （P 〈 0.05）, respectively; The transport stimulation didn＇t decreased the number of collected oocytes from the supemvulation lambs（P 〉 0.05）, however, the number of collected oocytes in the repeated superovulation group was reduced significantly compared with the control group （P 〈 0.05）; The embryonic culture medium containing 10 μmol/L EDTA eould improve significantly the development capability of in vitro produced embryo from lamb oocytes （P 〈 0.05）, and the healthy lambs were born by the embryo transfer.

关 键 词：幼羔 卵母细胞 胚胎 

分 类 号：S185[农业科学—农业基础科学]