小叶满江红内生细菌多样性的PCR-DGGE及电子显微镜分析  

Diversity Analysis of Endophytic Bacteria within Azolla microphylla Using PCR-DGGE and Electron Microscopy

作　　者：郑斯平[1] 陈彬[1] 关雄[2] 郑伟文[1] 

机构地区：[1]福建省农业科学院生物技术研究所,福州350003 [2]福建农林大学教育部生物农药与化学生物重点实验室,福州350002

出　　处：《农业生物技术学报》2008年第3期508-514,共7页Journal of Agricultural Biotechnology

基　　金：福建省科技计划项目(闽科计(2003)61号);福建省农科院青年人才创新基金项目资助

摘　　要：用16SrDNA-PCR-DGGE技术、电子显微镜和平板培养的方法系统地揭示了小叶满江红(Azolla microphylla)中内生细菌的遗传多样性和表型多样性。16SrDNA-PCR-DGGE的指纹图谱分析表明,在小叶满江红!蓝藻共生体内存在一个以蜡质芽胞杆菌(Bacillus cereus)为优势种群的复杂、多样的细菌区系;内生细菌在体内呈现各异的超微结构特征,体外培养生成不同大小、形态和色素的菌落。应用16SrDNA-PCR-DGGE技术能较全面地揭示满江红内生细菌的遗传多样性。Using 16S rDNA-PCR-DGGE, electron microscopy and conventional plating method, genetic diversity and phenotype polymorphism of the endophytic bacteria within Azolla microphylla were explored. 16S rDNA-PCR-DGGE profile showed that there was a complex and divergent bacterial community with Bacillus cereus as dominant species within Azolla-cyanobacteria association, which was supported by the fact that the endobacterial cells exhibited distinct ultmstructural characteristics in vivo and the bacteria appeared various colonies with different size, shape and colors in vitro. It indicated that the genetic diversity of endophytic bacteria in Azolla could be figured out by using 16S rDNA-PCR-DGGE.

关 键 词：满江红 内生细菌 多样性 16S rDNA-PCR-DGGE 

分 类 号：S188[农业科学—农业基础科学] S184