以病毒样颗粒为载体的小儿肾母细胞瘤新型疫苗的研制  

Immunogenicity of a novel vaccine with VLP as vector for Wilm＇s tumor

作　　者：王政[1] 段跃强[2] 罗德炎[2] 王希良[2] 刘洲禄[1] 

机构地区：[1]中国人民解放军总医院小儿外科,北京100853 [2]军事医学科学院微生物流行病学研究所免疫室

出　　处：《中华普通外科杂志》2008年第6期457-459,共3页Chinese Journal of General Surgery

基　　金：国家自然科学基金资助项目（30170853）

摘　　要：目的对小儿肾母细胞瘤以病毒样颗粒为载体的新型疫苗进行初步研究，为该肿瘤的治疗以及替代手术后放疗和化疗提供新的切入点。方法采用基因重组技术分别克隆编码小儿肾母细胞瘤WT1表位肽HLA-A＊2402、HLA-A＊2402x，将其融合到乙型肝炎核蛋白基因片段上，运用棒状杆菌表达系统对融合蛋白进行表达。结果细胞病变显示病毒重组，SDS-PAGE显示目的蛋白的表达，电镜下可观察到目的蛋白。结论小儿肾母细胞瘤WT1表位肽HLA-A＊2402、HLA-A＊2402x融合到乙型肝炎核蛋白基因片段上可以产生融合病毒样颗粒，该融合蛋白的制备将为小儿肾母细胞瘤的免疫治疗提供新的思路。Objective To develop a vaccine for Wilm＇s tumor in order to lay a foundation for effective treatment and substitute for the radiotherapy and chemotherapy of this tumor. Methods DNA fragments encoding the WT1 antigens（including, HLA-A ＊ 2402 and HLA-A ＊ 2402x）,and DNA fragments encoding the couple antigens HLA-A ＊ 2402-HBc and HLA-A ＊ 2402x-HBc were cloned and inserted into the eukaryotic expression vector pcDNA3.1 （ ＋ ） in sense orientation and baculovirus expression pFactbac respectively, then transfected into COS-7 cells and Sf 9 cell respectively. The expression of the target proteins in the cells were identified by CPE, SDS-PAGE and electron microscopy. Virus-like particles were observed under electron microscopy. Results The fusion gene of HLA-A ＊ 2402 and HLA-A ＊ 2402x and HBc express virus-like particle protein. Conclusions This preliminary result shows a hopeful future in developing an effective immunotberapy to Wilm＇s tumor.

关 键 词：肾母细胞瘤 病毒融合蛋白质类 疫苗 DNA 

分 类 号：R686[医药卫生—骨科学]