重组人甲状腺球蛋白检测TgAb间接ELISA法的建立及其初步应用  

Establishment of an Indirect ELISA Using Recombinant Human Thyroglobulin for Detecting TgAb and Its Preliminary Application

作　　者：李莎[1] 吕枚[1] 冯洁[1] 方佩华[1] 李宁[1] 许静[1] 杨立昌[1] 

机构地区：[1]天津医科大学总医院核医学科,300052

出　　处：《天津医药》2008年第6期401-403,共3页Tianjin Medical Journal

基　　金：天津市科技发展计划项目(项目编号:05YFGDSF02700)

摘　　要：目的:探讨用重组人甲状腺球蛋白(hTg)建立检测甲状腺球蛋白抗体(TgAb)的间接ELISA法及其临床意义。方法:用该法测定300例健康体检者(正常对照组)和各种甲状腺疾病患者484例;其中桥本甲状腺炎(HT)219例,Graves’病(GD)212例(初诊91例、复诊121例),非自身免疫性甲状腺疾病(AITD)患者53例。结果:确立本法正常界限值为0.74(A405值),血清TgAb水平HT组与正常对照组、非AITD组、GD组相比差异均有统计学意义(均P<0.01);GD组与正常对照组、非AITD组相比差异均有统计学意义(P<0.05或P<0.01);非AITD组与正常对照组相比差异无统计学意义(P>0.05)。HT、GD初诊、GD复诊患者的阳性率分别为70.8%、38.8%和28.6%,3类非AITD患者阳性率均不超过5.3%。结论:以重组hTg为抗原建立检测TgAb的ELISA法有助于各种甲状腺疾病的临床鉴别。Objective： To explore the method of indirect enzyme-linked immunosorbent assay （ELISA） with recombinant prokaryotic thyrogloblin for measuring TgAb and its clinical significance. Methods： Three hundreds health sera samples were measured using ELISA to verify cut-off values, and 484 sera samples from patients with thyroid diseases were measured with the same metod which included 219 HT,121 untreated GD,91 treated GD,and 53 nonautoimmune thyroid disease. Results： The cut-off value of TgAb in normal control group was 0.74 using ELISA method. The TgAb value in HT was higher than that in normal control group, non-AITD and GD groups, respectively. The TgAb value in GD was higher than that in normal and non-AITD groups. There was no significant difference in TgAb value between non-AITD and normal groups. The positive rates in HT, untreated GD,treated GD and non-AITD were 70.8%, 38.8%, 28.6% ,less than 5.3%, respectively. Conclusion： ELISA with recombinant hTg to detect TgAb can be used in identifying thyroid diseases.

关 键 词：甲状腺球蛋白 抗体 甲状腺炎 自身免疫性 格雷夫斯病 酶联免疫吸附测定 

分 类 号：R446.62[医药卫生—诊断学] R446.61[医药卫生—临床医学]