过表达Δ2Δfosb蛋白促进小鼠原代前成骨细胞的分化(简报)  

OVEREXPRESSION OF THE TRUNCATED fosb ISOFORM (Δ2Δfosb) INCREASES NODUS IN CD1 PRIMARY OSTEOBLASTES

作　　者：郑惠玲[1] 袁超[1] 包黎娟[1] 安俊辉[1] 祝珍珍[1] 杨公社[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《分子细胞生物学报》2008年第3期222-226,共5页Journal of Molecular Cell Biology

基　　金：国家重点基础研究发展计划(973计划)(2004CB117506);西北农林科技大学人才专项基金(编号01140502)

摘　　要：小鼠骨肉瘤基因B（Finkef—Biskis—Jinkins murine osteosarcoma B，fosb）是具亮氨酸拉链结构的转录因子激活剂蛋白-1（activator protein-1，AP-1）家族的成员之一。fosb基因在转录拼接过程中会自发地发生剪切截去C端的101个富含脯氨酸的氨基酸区域而形成△fosb，被截断的区域包括转录激活结构域，The recombinant adenoviruses which can overexpress fosb and △2△fosb proteins were produced. Primary calvarial osteoblasts from new born CD1 mice were infected with those adenoviruses by different proportion of Ad-△2△fosb and induced by 5 mmol/L β-glycerophosphate, 50 μg,/ml ascorbic acid and 10 nmol/L dexamethasone for 23 days and 29 days, respectively. The re- sults by Alizarin Red Staining showed that the primary osteoblasts overexpressing △2△fosb protein formed more nodus than the control cells and the nodus in overexpressing △2△fosb protein osteoblasts were smaller than those in control cells on day 23. Differentiation of the primary osteoblasts overexpressing △2△fosb protein increased more than that of the control cells on day 29. It is an evidence for the reason that bone density of △2△fosb transgenic mice was markedly increased throughout the skeleton in new born and mature mice.

关 键 词：成骨细胞 分化 FOSB 茜素红染色 

分 类 号：Q343[生物学—遗传学]