西藏藏族人群15个短串联重复序列基因座的遗传多态性  

Genetic polymorphism of 15 STR loci in Tibetan population

作　　者：李宁[1] 苏玉虹[2] 席焕久[3] 任甫[3] 黄克强[4] 肖艳杰[1] 温有峰[3] 李春山[4] 李长勇[3] 王鲲[4] 成建国 

机构地区：[1]辽宁医学院基础学院,锦州121001 [2]辽宁医学院动物科学院,锦州121001 [3]辽宁医学院人类学研究所,锦州121001 [4]辽宁医学院附属第二医院,锦州121001 [5]西藏自治区拉萨市第二人民医院内科,拉萨851000

出　　处：《遗传》2008年第7期851-856,共6页Hereditas（Beijing)

基　　金：国家自然科学基金项目(编号:30270696);辽宁省教育厅基金项目(编号:202172050,2004C038)资助~~

摘　　要：利用多重PCR五色荧光（6FAM、VIC、NED、PET、LIZ）自动化检测技术检测西藏自治区藏族人群DSS1179、D21S11、D7S820、CSF1PO、D3S1358、TH01、D13S317、D16S539、D2S1338、D19S433、VWA、TPOX、D18S51、D5S818及FGA共15个STR基因座遗传多态性，获得15个STR基因座的群体遗传学数据。结果显示：15个STR基因座的基因型分布符合Hardy-Weinberg平衡。15个STR基因座的个体鉴别力（Discrimination power,DP）在0．7555～0．9602之间，杂合度（Heterozygosity,H）在0．5651～0．8530之间，多态性信息含量（Polymorphism information content，PIC）在0．5528～0．8456之间，非父排除率（Probability of paternity exclusion，EPP）在0．3811～0．8549之间，累积个体鉴别力为0．999999999，累积非父排除率为0．999999998。15个短串联重复序列基因座适合作为西藏藏族人群的遗传标记，用于人类学、疾病连锁分析、法医学亲子鉴定和个体识别等领域的研究。The polymorphism distributions of 15 STR loci （D8S1179, D21S11, D7S820, CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, D5S818, and FGA ） were investigated in a Tibetan population by multiplex PCR amplification using five fluorochromes （6FAM, VIC, NED, PET, LIZ）. Gene frequency, discrimination power （DP）, heterozygosity （H）, polymorphism information content （PIC） and probability of paternity exclusion （EPP） were calculated, and all loci were tested for Hardy-Weinberg equilibrium. Results indicate that the gene frequency of these 15 STR loci is in Hardy-Weinberg equilibrium. The DP is at 0.7555-0.9602, H is at 0.5651-0.8530, PIC is at 0.5528-0.8456, and EPP is at 0.3811-0.8549. Cumulative DP of the 15 STR is 0.99999999, and cumulative EPP is 0.999999997. Therefore, these 15 STR loci can be used as genetic markers of Tibetan populations in anthropological studies, linkage analysis of genetic diseases, individual identification and paternity testing in forensic medicine.

关 键 词：短串联重复序列 多态性 藏族 聚合酶链式反应 

分 类 号：Q987[生物学—遗传学] R394[生物学—人类学]