长爪沙鼠的遗传多样性分析  

Genetic diversity of Mongolian gerbils(Meriones unguiculatus)

作　　者：丁贤明[1] 钱宝珍[1] Matsuda Junichiro Koura Minako 萨晓婴[3] 施张奎[3] 

机构地区：[1]浙江省医学科学院生物工程研究所,杭州310013 [2]日本国家实验动物中心,大阪 [3]浙江省医学科学院实验动物中心,杭州310013

出　　处：《遗传》2008年第7期877-884,共8页Hereditas（Beijing)

基　　金：浙江省自然科学基金课题(编号:Y204004)资助~~

摘　　要：利用17个微卫星DNA标记对Z：ZCLA长爪沙鼠封闭群、野生群和近交系进行遗传多样性分析，评估群体内的遗传变异和群体间的遗传分化。结果表明：在Z：ZCLA封闭群和野生群中共有9个微卫星DNA标记获得稳定的结果，分别为AF200940、AF200941、AF200942、AF200945、AF200946、AF200947、D11Mit128、PKC和SCN，共检测到41个等位基因，每个基因的等位基因数从1～7不等，片段大小在120～283bp之间，所有位点的平均期望杂合度（He）和多态信息含量（PIC）值分别为0．5032和0．4656，Z：ZCLA封闭群和野生群9个微卫星位点平均有效等位基因数分别为2．78和2．89，平均基因杂合度分别为0．3704和0．3893，平均多态信息含量分别为0．3256和0．3344，两个群体都表现为中度多态，Z：ZCLA封闭群较野生群稍低；在3个近交系中共有8个位点获得稳定的扩增结果，分别为AF200941、AF200942、AF200945、AF200946、AF200947、D11Mit128、PKC和SCN，共检测到11个等位基因，片段大小在140～241bp之间，其中5个位点在群体内表现为单态纯合，3个位点在群体内表现为单态杂合，所有位点在群体内和群体间均呈单态性，表明这3个长爪沙鼠品系基本符合近交系的要求，微卫星标记技术适用于近交系长爪沙鼠的遗传检测。Genetic diversity of Z：ZCLA Mongolian gerbils, wild Mongolian gerbils and 3 inbred M. gerbil strains was evaluated with 17 microsatellite loci. The genetic variabilities within and between populations were estimated. The results showed that 9 microsatellite DNA, AF200940, AF200941, AF200942, AF200945, AF200946, AF200947, DllMit128, PKC, and SCN, were amplified efficiently both in Z：ZCLA M. gerbils and the wild M. gerbils. Forty-one alleles were amplified with the number of alleles per locus ranging from 1 to 7. The average expected heterozygosity （He） and polymorphism information content （PIC） of all the loci were 0.5032 and 0.4656, respectively. The mean effective allele number of Z：ZCLA M. gerbils and wild M. gerbils were 2.78 and 2.89. The PIC of Z：ZCLA M. gerbils and the wild M. gerbils were 0.3704 and 0.3893. In the 3 inbred M. gerbils strains, 8 microsatellite DNA were amplified efficiently with 11 alleles. It displayed het- erozygosity in AF200941, AF200945, AF200946, DllMit128, and SCN loci with fragment lengths from 140 to 215 bp; and homozygosity in AF200942, AF200946, and AF200947 with fragment lengths from 203 to 241 bp. All of the 8 microsatel- lite loci were monomorphic both within and among the strains. These results suggested that the moderate genetic diversity of the conventional closed colony of Z：ZCLA M. gerbils was observed; and inbred M. gerbils strains basically met the request. Microsatellite markers can be used in monitoring of M. gerbils populations.

关 键 词：微卫星DNA 长爪沙鼠 遗传监测 

分 类 号：Q953[生物学—动物学]