MC4R、POU1F1基因对京海黄鸡生长性能的遗传效应  

Genetic effects of MC4R and POU1F1 gene on the growth performance in Jinghai yellow chicken

作　　者：陶勇[1,2] 李国辉[1] 胡玉萍[1] M. Dafalla Mekki 陈宽维[4] 王金玉[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]江苏畜牧兽医职业技术学院,泰州225300 [3]苏丹科特凡大学自然资源和环境研究院动物科学系,苏丹北科特凡州51111 [4]中国农业科学院家禽研究所,扬州225003

出　　处：《遗传》2008年第7期900-906,共7页Hereditas（Beijing)

基　　金：国家发改委高技术产业化专项资金(编号:2007-37);江苏省高新技术项目(编号:BG2004316);江苏省“青蓝工程”培养基金资助~~

摘　　要：以MC4R和POU1F1基因为候选基因,采用PCR-SSCP和DNA测序技术检测两个候选基因在京海黄鸡群体中的单核苷酸多态性(SNPs),同时对候选基因与京海黄鸡生长性能的相关性进行了研究。结果表明,MC4R基因编码区第662bp位置有G→C碱基的点突变,在京海黄鸡中检测到AA、AB、BB3种基因型,A等位基因频率为0.929,B等位基因频率为0.071;在POU1F1基因exon3在序列的第5231bp位置有一个A→T碱基的点突变,检测到CC、CD、DD3种基因型,C等位基因频率为0.500,D等位基因频率为0.500。采用GLM模型分析基因型对生长性能的遗传效应,结果表明,MC4R基因AA基因型个体的4、8、12周龄体重显著地高于BB型个体(P<0.05),16周龄体重差异极显著(P<0.01);POU1F1基因CD基因型个体体重极显著高于CC型和DD型(P<0.01)。因此推测MC4R和POU1F1基因可能是影响鸡生长性状的主效基因或与主效基因紧密连锁的标记基因,能够在分子标记辅助选择中用于对鸡生长性状的遗传改良。Melanocortin-4 receptor （MC4R） gene and pituitary specific transcription factor 1 （POU1F1） gene were studied as candidate genes on the Jinghai yellow chicken in this study. Polymorphisms of MC4R and POU1F1 gene had been analyzed by PCR-SSCP and DNA sequencing method. The relationship between two candidate genes and the growth performance of Jinghai yellow chicken had also been analyzed by GLM model. The frequency of A/B alleles for MC4R gene in the population was 0.929 and 0.071, and that of C/D alleles for POU1F1 gene was 0.500 and 0.500. The results showed that AA genotype of MC4R gene had higher bodyweight at 4, 8, 12 weeks compared to AB and BB genotype （P〈0.05）; CD genotype of PO U1F1 gene had significantly higher bodyweight at all weeks compared to CC and DD genotype （P〈0.01 ）. So, it could be preliminarily deduced that MC4R and POU1F1 gene were probably the major gene on growth performance or a QTL linked gene which associated with growth performance in chicken.

关 键 词：京海黄鸡 MC4R基因 POU1F1基因 PCR—SSCP 生长性状 

分 类 号：S831[农业科学—畜牧学]