aFGF和TGFβ1联合诱导猪牙乳头细胞向成牙本质样细胞分化  

Differentiation of porcine dental papillae cells into odontoblast-like cells by combination of aFGF and TGFβ1

作　　者：余姗姗[1,3] 翁雨来[2] 蒋欣泉[3] 

机构地区：[1]上海交通大学附属第一人民医院口腔科,上海200080 [2]上海交通大学医学院附属第九人民医院口腔内科,上海200011 [3]上海市口腔医学重点实验室,上海市口腔医学研究所,上海200011

出　　处：《口腔医学》2008年第6期281-283,294,共4页Stomatology

基　　金：国家重点基础研究发展规划973项目(G1999054308)

摘　　要：目的建立猪牙乳头细胞向成牙本质样细胞分化的体外诱导方案。方法联合应用aFGF和TGFβ1对体外培养的猪牙乳头细胞进行平面诱导,观察诱导后细胞的形态学变化,通过Von Kossa染色检测诱导后细胞的矿化能力,并采用免疫荧光染色和RT-PCR检测成牙本质细胞特异性标志物——DSP蛋白和DSPP mRNA在诱导后细胞中的表达。结果诱导后部分细胞出现细长的单侧细胞突起,在矿化液培养2周后形成典型的矿化结节,并且表达DSP蛋白和DSPP mRNA。结论联合应用aFGF和TGFβ1可以诱导体外培养的猪牙乳头细胞向成牙本质样细胞分化。Objective To induce porcine dental papillae cells to differentiate into odontoblast-like cells in vitro. Methods The cultured porcine dental papillae cells（pDPCs） were induced by aFGF（25 ng/ml） ＋ TGFβ1 （10 ng/ml）. Cell morphology after induction was observed. Mineralization capability of the induced cells was evaluated by Van Kossa staining. The expressions of DSP protein and DSPP mRNA were detected by immunofluorescent staining and RT-PCR. Results Part of the induced cells showed a long single process. Classic mineralized nod- ules were found in the induction cultures after 2 weeks. DSP protein and DSPP mRNA were expressed in cells induced by aFGF ＋ TGFβ1. Conclusion aFGF ＋ TGFβ1 can induce pDPCs to differentiate into odontoblast-like cells.

关 键 词：牙乳头细胞 酸性成纤维细胞生长因子 转化生长因子β1 分化 

分 类 号：R329.21[医药卫生—人体解剖和组织胚胎学]