肝星状细胞SSeCKS的表达及其在肝纤维化中的作用  

The role of hepatic stellate cells SSeCKS expression in liver fibrosis

作　　者：蒋文[1] 宋磊[2] 邵建国[1] 王陆军[1] 陆建荣[1] 刘海鸥[3] 

机构地区：[1]南通市第三人民医院消化科,江苏省南通市226006 [2]南通市第一人民医院儿科 [3]南通大学免疫实验室

出　　处：《中华内科杂志》2008年第7期570-573,共4页Chinese Journal of Internal Medicine

基　　金：南通市社会发展科技计划项目（S2006047）

摘　　要：目的了解src抑制的蛋白激酶C底物（SSeCKS）在肝星状细胞（HSC）活化中的变化和作用。方法（1）分离正常大鼠的HSC，以实时定量PCR检测其在体外培养过程中SSeCKS mRNA的表达变化，以蛋白质印迹法和细胞免疫荧光法检测HSC中SSeCKS蛋白的表达变化。（2）制作肝纤维化的大鼠模型，以免疫荧光法检测肝组织内SSeCKS和α-平滑肌肌动蛋白（α-SMA）的表达变化及定位。结果初分离的HSC表达低水平的SSeCKS mRNA，经培养活化后SSeCKS的表达增强。在纤维化肝组织中，SSeCKS阳性细胞增多，阳性细胞多分布于肝窦周围，与α-SMA阳性细胞的分布一致。结论在体外活化过程中HSC中SSeCKS表达增强。SSeCKS可能参与HSC在肝脏炎症和纤维化中的作用。Objective To investigate the change and effect of SSeCKS （ src suppressed c kinase substrates） in the activation of hepatic stellate cells （HSCs）. Methods HSCs were isolated from normal rats, the change of SSeCKS mRNA expression on HSCs culture in vitro was determined using real-time PCR, protein level was determined by Western blot and immunofluorescence methods. A rat model of liver fibrosis was established. The expression and location of SSeCKS and α-SMA （ α-smooth muscle actin） in liver tissues were detected by immunofluorescence methods. Results SSeCKS mRNA expression was low in freshly isolated HSCs cell and the expression increased in activated HSCs in vitro. In liver fibrosis tissue, the number of SSeCKS-positive cells was increased and these cells were distributed along the sinusoids which also contained α-SMA positive cells. Condusion The expression of SSeCKS was increased in activated HSCs in vitro. Therefore, SSeCKS may be involved in the liver inflammation and fibrosis.

关 键 词：肝纤维化 肝星状细胞 src抑制的蛋白激酶C底物 

分 类 号：R686[医药卫生—骨科学]