槲寄生凝集素抑制人大肠癌HCT116细胞环氧化酶-2表达的研究  

Inhibitive effect of mistletoe lectins on cyclooxygenase-2 expression in colon cancer HCT116 cell line

作　　者：林蕾[1] 叶孟[2] 王少敏[2] 倪曙民[2] 吴骁[2] 

机构地区：[1]宁波大学医学院附属医院皮肤科 [2]宁波大学医学院附属医院肿瘤科

出　　处：《中国临床药理学与治疗学》2008年第5期504-507,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：宁波市博士基金资助项目(2005A610020);宁波市医学科研项目(200529)

摘　　要：目的:探讨槲寄生凝集素对人大肠癌HCT116细胞环氧化酶(COX-2)表达的影响。方法:采用RT-PCR、Western blotting和ELISA等方法检测不同浓度(0.1～1mg/L)槲寄生凝集素对细胞COX-2及其产物前列腺素E2(PGE2)表达的影响。结果:槲寄生凝集素可抑制HCT116细胞的COX-2mRNA、蛋白表达及PGE2的水平,其抑制COX-2蛋白的表达与PGE2的水平相一致,只在大剂量(1mg/L)时对COX-1mRNA有抑制作用。结论:槲寄生凝集素具有抑制HCT116细胞的COX-2基因转录、蛋白表达和功能活性等作用,在一定浓度(0.1～1mg/L)和时间范围(3～24h)内,表现出时效与量效关系。AIM： To investigate the effect of mistletoe lectins on cyelooxygenase-2 （COX-2） expression in colon cancer HCT116 cells at the level of gene and protein. METHODS： Levels of mRNA and protein expression of COX-2 and its synthesized product prostaglandin E-2 （PGE2） of colon cancer HCT116 cell line were detected by RT-PCR , Western blotting , ELISA respectively before and after treatment of mistletoe leetins in different concent rations. RESULTS： Mistletoe leetins had a remarkable inhibitory effect on the mRNA and protein expression of COX-2, and synthesis of PGE2 in colon cancer HCT116 cell line, but had effect on COX-1 only at large dose. CONCLUSION： Mistletoe lectins has selective inhibitory effect on gene transcription, protein expression and functional activity of COX-2 in HCT116 cell line, which is time-dependent and concentration-dependent within certain range of concentration and acting time.

关 键 词：槲寄生凝集素 大肠癌HCT116细胞系 环氧化酶-2 前列腺素E2 

分 类 号：R965.2[医药卫生—药理学]